Integrated Rapid Mixing Apparatus for CD, Fluorescence, Absorbance, Raman, Chemical Quench and Freeze Quench

这项提议将为一个综合的快速混合系统提供资金，该系统将允许进行停流、连续流、冻结淬火和化学淬火实验。在停流或连续流动模式下，可以用吸收、荧光或拉曼散射和圆二色谱来研究反应。在化学和冷冻淬火模式下，活性中间体可以被捕获，然后通过电子顺磁共振、核磁共振或其他光谱分析进行表征。项目将由来自生物化学、生理和生物物理系的六个主要用户进行。该仪器还将向其他几个用户提供，并将成为NIH支持的分子生物物理学培训计划的一部分。总的来说，该仪器将应用于蛋白质和核酶折叠、几种酶底物相互作用、血红蛋白中的配体结合、蛋白质-DNA相互作用和蛋白质易位的研究。

This proposal will provide funds for an integrated rapid mixing system that will allow stopped flow, continuous flow, freeze quench and chemical quench experiments to be carried out. In the stopped or continuous flow modes reactions can be studied by absorption, fluorescence or Raman scattering and circular dichroism. In the chemical and freeze quench modes reactive intermediates can be trapped and then characterized by electron paramagnetic resonance, nuclear magnetic resonance, or other spectroscopies. Projects will be conducted by six major users from the Departments of Biochemistry and Physiology and Biophysics. The instrumentation will also be available to several additional users and will be part of a NIH supported Training Program in Molecular Biophysics. In general, the instrumentation will be applied to the study of protein and ribozyme folding, several enzyme substrate interactions, ligand binding in hemoglobins, protein-DNA interactions and protein translocation.