CAREER: Immuno-Optical Biosensors for Plasma Factors/ Application of TQM in Bioengineering Education

9733207康蛋白C(PC)是一种重要的血浆蛋白，它可以防止不需要的血栓，也可以溶解人体内形成的血栓。PC缺乏症，无论是遗传性的还是后天性的，人体都会遇到严重的血液凝固并发症，这可能会导致血栓，损害肺、心脏和脑组织，并可能导致死亡。一种实用的、临床上诊断PC缺乏症的PC生物传感器应具有高灵敏度、高特异性和实时测量的属性。所提出的机制依赖于高度特异的抗原-抗体反应。它包括在光纤表面结合抗体，将样本注入包裹光纤的微腔(300微升)，使发光二次抗体与纤维上吸收的PC分子反应，并通过发射的光强度定量PC浓度。建议将全面质量管理(TQM)理念应用于生物工程研究生和本科生的教育和研究。该项目强调结构化的团队方法，将基础科学家、生物工程师(生物医学和生物过程工程师)、临床医生、商业和法律专业人员聚集在一起，以最低成本生产最佳生物产品的最佳过程开发。***

9733207 Kang Protein C (PC) is an important blood plasma protein which prevents unwanted clots and also dissolves formed clots in the human body. With PC deficiency, either by hereditary or acquired, the human body can encounter serious medical complications due to blood coagulation that can lead to embolisms that injure lung, heart, and brain tissue, and can cause death. A practical, clinical PC biosensor for the diagnosis of PC deficiency should have the attributes of high sensitivity, high specificity, and real-time measurement. The proposed mechanism depends on a highly specific antigen-antibody reaction. It involves binding antibodies at the surface of an optical fiber, injection of the sample into a micro chamber (300 micro liter) encasing the fiber, reacting light emitting secondary antibodies with the absorbed PC molecules on the fiber, and quantifying the PC concentration by the emitted light intensity. It is proposed to apply total quality management (TQM) concepts in both graduate and undergraduate bioengineering education and research. This project emphasizes the structured team approach to bring together basic scientists, bioengineers (biomedical and bioprocess engineers), clinicians, business, and legal professionals to address optimum process development for the production of the best bioproduct at minimum cost. ***