POWRE: Identification of Auxin Signal Transduction Mutants in Arabidopsis

9805787穆菲特这项研究计划将使用一种新的、成熟的遗传学方法来研究生长素信号转导途径。生长素是一种重要的植物生长激素，100多年来一直是人们深入研究的对象。然而，这种重要的生长物质发挥作用的机制仍然不清楚。这项拟议的研究几乎肯定会为理解生长素的作用提供重要的见解。这项工作利用了PI构建的许多新的转基因植物系，以使用生长素特定启动子控制下的报告基因。对转基因植株进行诱变，筛选出报告基因表达发生变化的突变株系。几个新的突变体已经被分离出来，拟议的研究将导致对突变植物的详细描述，并最终克隆这些基因。由于生长素在植物生长发育中的核心作用，识别生长素信号转导相关基因将对植物生物学研究具有重要意义，并最终可能产生控制作物生产的新方法。拟议的研究还将使PI能够制定一个出色的长期研究项目，证明她有能力成为一名独立的研究员，并使她在寻找长期教职时具有很强的竞争力。

9805787 Murfett This POWRE proposal will investigate the auxin signal transduction pathway using a novel and well developed genetic approach. Auxin, which is an essential plant growth hormone, has been the subject of intensive research for over 100 years. However, the mechanisms by which this essential growth substance functions remain unknown. The proposed research will almost certainly provide important insights for understanding auxin action. The work utilizes a number of novel transgenic plant lines that the PI has constructed to use a reporter gene that is under the control of an auxin-specific promoter. The transgenic plants were mutagenized and mutant lines that display altered expression of the reporter gene were isolated. Several novel mutants have already been isolated and the proposed research will result in detailed characterization the mutant plants and eventually cloning of the genes. Because of the central role of auxin in plant growth and development, identifying the genes involved in the transduction of auxin signals will be of fundamental significance to plant biology research and may eventually result in novel methods for controlling crop production . The proposed research will also allow the PI to develop an excellent long-term research project that will demonstrate her ability to be an independent researcher and make her very competitive when searching for permanent faculty positions.