Instrumentation for Infared and Raman Spectroscopy Facility

化学研究仪器和设施计划(CRIF)的这一奖项将帮助北卡罗来纳州立大学化学系获得纳秒时间分辨率、带FTIR显微镜的步进扫描傅里叶变换红外(FTIR)光谱仪工作台和衰减全反射(ATR)附件。分步扫描FTIR光谱将用于观察新型脱卤酶中的酶中间体和铁硫树枝状大分子中伴随电子转移的结构动力学，确定底物与酶结合位置相互作用的时间尺度，以及卤型微孔柔性骨架的构象变化。ATR模式下的FTIR将用于测量不透明晶体和聚合物的振动光谱。傅里叶变换红外光谱(FTIR)近年来已成为一种非常强大的工具。干涉仪稳定性和重现性的提高显著提高了FTIR光谱仪的灵敏度。新的FTIR样品几何形状，包括掠入射和衰减全反射光谱，增加了样品的种类，包括不透明的晶体、表面和聚合物。

This award from the Chemistry Research Instrumentation and Facilities Program (CRIF) will assist the Department of Chemistry at North Carolina State University to acquire a nanosecond time resolution, step-scan Fourier-transform infrared (FTIR) spectrometer bench with FTIR microscope and attenuated total reflection (ATR) attachment. Step-scan FTIR spectroscopy will be used to observe enzyme intermediates in novel dehalogenating enzymes and structural dynamics that accompany electron transfer in iron-sulfur dendrimers, to determine time scales for substrate interactions with enzyme binding sites and for conformation changes in the flexible framework of halozeotype micropores. FTIR in ATR mode will be used to measure the vibrational spectrum of opaque crystals and polymers. Fourier-transform infrared (FTIR) spectroscopy has become a very powerful tool in recent years. Improvements in the stability and reproducibility of interferometers have resulted in significantly improved sensitivity in FTIR spectrometers. New FTIR sample geometries, including grazing incidence and attenuated total reflection spectroscopy, have increased the repertoire of samples to include opaque crystals, surfaces and polymers.