Characterization of Hematopoietic Cell Cultures

这项建议是为了研究骨髓干细胞体外扩增为成熟血细胞的关键医学领域，这些细胞可用于各种治疗，例如用于抗感染的中性粒细胞、用于凝血的血小板等。研究小组包括生物过程工程师、计算机建模专家和骨髓移植方面的内科专家，将尝试使用代谢分析(细胞签名)来表征这些复杂的培养。该小组最近开发了一个数学模型，可以区分影响红细胞培养的假设，并计划将该模型扩展到粒细胞和巨核细胞系。为了实现这些目标，该小组将广泛研究细胞增殖、祖细胞含量、分化标记物(例如，表面抗原表达)和细胞代谢。

This proposal is to study the critical medical area of ex-vivo expansion of bone-marrow stem cells into mature blood cells useful for a variety of therapies, e.g., neutrophils to fight infection, platelets for clotting, etc. The research team, which includes bioprocess engineers, a computer-modeling expert, and a physician expert in bone-marrow transplantation, will attempt to employ metabolic assays (cell-signatures) to characterize these complex cultures. The group has recently developed a mathematical model that can discriminate between hypotheses effecting red blood cell cultures and plans to extend this model to granulocytes and megakeryocyte lineages. In order to achieve these goals, the group will extensively characterize cell proliferation, progenitor cell content, differentiation markers (e.g., surface antigen expression), and cell metabolism.