Structure and function of the membrane insertase YidC

膜蛋白的一个关键特征是，除了合成外，它们还必须在膜中正确组装。在细菌中，这种膜蛋白组装过程由内膜蛋白YidC催化。YidC是一种进化上保守的膜蛋白，也存在于线粒体和叶绿体膜中。YidC可以作为单一酶或与SecYEG易位通道协同工作，SecYEG易位通道将大多数蛋白质插入E.杆菌关于YidC的未回答的问题是：这种蛋白质如何在膜生物发生过程中与底物结合，它如何组装跨膜片段以促进膜蛋白的折叠，以及这种膜蛋白插入酶的分子结构是什么？该提议有三个主要目标：（1）定义底物结合口袋，（2）确定Sec依赖性蛋白质的YidC功能和（3）定义YidC的分子结构。解决这些方面将提供重要的信息YidC如何作为一个网站的插入和组装的膜蛋白。

A key feature of membrane proteins is that, in addition to being synthesized, they must assemble properly in the membrane. In bacteria, this membrane protein assembly process is catalyzed by the inner membrane protein YidC. YidC is an evolutionarily conserved membrane protein also found in mitochondrial and chloroplast membranes. YidC can work as a single enzyme or in concert with the SecYEG translocation channel that inserts most proteins into the inner membrane in E. coli. Unanswered questions regarding YidC are: how does this protein bind to substrates during membrane biogenesis, how does it assemble transmembrane segments to promote folding of membrane proteins, and what is the molecular structure of this membrane protein insertase? This proposal has three main goals: (1) defining the substrate binding pocket, (2) determining the YidC function for Sec-dependent proteins and (3) defining the molecular structure of YidC. Solving these aspects will provide important information on how YidC functions as a site for insertion and assembly of membrane proteins.