Doctoral Dissertation Research: Mitochondrial DNA Sequence Variation of Ancient Eastern North American Arctic Peoples

“博士论文研究：古代北美东部北极民族的线粒体DNA序列变异”这里讨论的问题与古爱斯基摩人多塞特和新爱斯基摩人图勒文化之间的基因混合有关。当图勒文化在加拿大北极地区传播时，这些文化的承载者之间是否有接触？或者，多塞特人在图勒文化到达加拿大北极之前就消失了？此外，谁是南安普顿岛的萨德尔米特？为了回答这些问题，将从北美东部北极考古遗址挖掘的骨骼材料中提取古代DNA，这些考古遗址横跨加拿大哈德逊湾和昂加瓦湾西海岸，一直延伸到格陵兰岛。利用聚合酶链式反应（PCR）从古代DNA样本中扩增线粒体基因组的高可变片段1 （HVS 1）并测序。成对序列差异将用于系统发育分析，以评估多塞特、图勒和萨德尔米特文化人群之间的遗传关系。研究结果将解决目前关于史前多塞特文化和图勒文化成员之间进化关系的争论，并评估萨德勒米特人是否与多塞特文化、图勒文化或现存的因纽特人群体关系更密切。

"Doctoral Dissertation Research: Mitochondrial DNA Sequence Variation of Ancient Eastern North American Arctic Peoples"The questions addressed here have to do with the genetic admixture between the people of the Paleoeskimo Dorset and Neoeskimo Thule cultures. Was there contact between the bearers of these cultures as the Thule culture spread across the Canadian Arctic? Or, alternatively, had the Dorset bearers disappeared before the Thule culture arrived in the Canadian Arctic? Further, who where the Sadlermiut of Southampton Island? To answer these questions, ancient DNA will be extracted from skeletal material excavated at Eastern North American Arctic arhaeological sites spanning the West coast of Hudson Bay and Ungava Bay in Canada to Greenland. The hypervariable segment 1 (HVS 1) of the mitochondrial genome will be amplified from the ancient DNA samples using the Polymerase Chain Reaction (PCR) and sequenced. Pairwise sequence differences will be used in phylogenetic analyses to assess the genetic relationship between peoples of the Dorset, Thule, and Sadlermiut cultures. The results will address the current debate over the evolutionary relationships between members of the prehistoric Dorset and Thule cultures and assess whether the Sadlermiut are more closely related to the Dorset, Thule, or living Inuit groups.