A census of viruses through the drinking water cycle

水生病毒具有很高的公共卫生意义，但迄今为止被低估了。在水监测和监督条例中，病毒检测迄今为止不是强制性的。这反映在现有的方法汇编中。迄今为止，用于检测不同类型的沃茨（废水、地表水、地下水、饮用水）中的各种类型的病毒的方法不够灵敏。一些最重要的水性病毒，如诺如病毒，只能通过PCR方法检测。在水媒病毒爆发的情况下，由于缺乏可靠的检测方法，往往无法澄清根本的情况和原因。这同样适用于生物犯罪或恐怖主义行为。因此，最重要的是进一步开发用于在不同类型的沃茨中进行灵敏和可靠的病毒检测的方法，这些方法在技术上容易在短时间内完成，在小体积中提供足够浓度的大范围病毒，具有高病毒回收率，不会太昂贵，并且将提供可再现的结果。在本提案中，将与各个项目伙伴合作开发浓缩大量水的方法，通过这些方法可以同时检测水样中的大量病毒。在实验室成功开发和测试后，将评估这些方法在不同沃茨和水处理步骤中的使用，以进行定量和定性病毒分析。

Waterbome viruses have a high but so far underestimated public health significance. In water monitoring and surveillance regulations, virus detection is until now not mandatory. This is reflected in the methodological repertoire available. To date, methods for detecting the various types of viruses in different types of waters (waste water, surface water, groundwater, drinking water) are insufficiently sensitive. Some of the most important waterborne viruses like noroviruses can only be detected by PCR methods. In the case of waterborne virus outbreaks, underlying circumstances and causes frequently cannot be clarified in the absence of reliable detection methodology. The same would apply to acts of biological crime or terrorism. It is thus of utmost importance to further develop methods for sensitive and reliable virus detection in different types of waters which are technically easy to accomplish in a short time, provide a sufficient concentration of a large range of viruses in a mall volume, have a high virus recovery rate, will not be too costly, and will deliver reproducible results. In this proposal methods for concentrating large volumes of water by which a large spectrum of viruses can be simultaneously detected in water samples will be developed in cooperation with individual project partners. After successful development and testing in the lab, the methods will be evaluated for its use in different waters and water treatment steps for quantitative and qualitative virus analysis.