A Multi-User, Research and Training Ultracentrifugation Facility

本提案是为了在约翰霍普金斯大学霍姆伍德校区建立一个分析超离心机设施。最初，该设施将支持生物化学，生物物理学和细胞与分子生物学领域的12名教员的研究。使用该设备分析分子组件的流体动力学特性将与校园生物热量计中心的这些小组进行的工作高度互补。使用该设备的研究将包括组蛋白和核小体的研究；重复蛋白在信号转导中的作用；半乳糖抑制因子；分子靶向与药物设计的新策略RNA二十面体植物病毒的调控相互作用植物木脂素对HIV-1和HSV-1感染的控制作用以及葡萄球菌核酸酶的低聚物。

This proposal is for the establishment of an analytical ultracentrifuge facility on the Homewood campus of Johns Hopkins University. Initially this facility will support the research of 12 faculty members in the areas of Biochemistry, Biophysics and Cell and Molecular Biology. The use of this facility for the analysis of the hydrodynamic properties of molecular assemblies will be highly complementary with the work carried out by these groups at the Biocalorimeter Center on campus. Research to use the facility will include studies of histones and nucleosomes; repeat-protein functions in signal transduction; the galactose repressor; new strategies for molecular targeting and drug design; regulatory interactions in RNA icosahedral plant viruses; control of HIV-1 and HSV-1 infection by plant lignans; and oligomers of staph nuclease.