A BAC Library Resource for Crop Genomics

克莱姆森大学建立了一个基因组研究所(CUGI)，专注于农业基因组学的研究、教学和服务。CUGI的服务部分是从植物和植物病虫害中生产高质量的细菌人工染色体(BAC)文库，并提供方便和负担得起的获取这些产品的途径。在几个高通量机器人仪器的帮助下，CUGI已经制作、获取和分发了当今使用的大多数植物和真菌BAC文库。由于对中央地理信息系统服务部分的需求日益增加，因此需要资金支持该服务设施的运作。这一项目的资助将扩大CUGI提供的服务，包括：EST(表达序列标签)克隆的挑选、排列和重新排列；BAC和EST DNA的排列和重新排列到过滤器上；产生BAC DNA池，用于用引物筛选文库；BAC和EST克隆的分发；通过杂交和聚合酶链式反应筛选BAC文库；通过杂交和聚合酶链式反应鉴定BAC克隆的指纹；BAC末端测序；关于BAC指纹、杂交和BAC末端序列数据的中央数据库；通过在克莱姆森举办的研讨会，培训BAC文库的构建和表征；开发用于基因表达谱分析的微阵列技术，并将这项技术提供给社区。CUGI作为一个非营利性组织运作，并将在收回成本的基础上提供这些服务。此处所述的CUGI服务部分应在拟议的供资期末完全自给自足。

Clemson University has established a Genomics Institute (CUGI) focusing on research, teaching and service in agricultural genomics. The service component of the CUGI has been to produce high-quality bacterial artificial chromosome (BAC) libraries from plants and plant pests and to provide convenient and affordable access to these products. With the assistance of several high-throughput robotic instruments, the CUGI has produced, acquired and distributed the majority of plant and fungal BAC libraries in use today. Because of the increasing demand on the service component of CUGI, funding is requested to support operations of the service facility. Funding of this project will expand services provided by CUGI to include: picking, arraying and rearraying of EST (expressed sequence tag) clones; arraying and rearraying of BAC and EST DNA onto filters; production of BAC DNA pools for library screening with primers; distribution of BAC and EST clones; BAC library screening by hybridization and PCR; fingerprinting of BAC clones identified by hybridization and PCR; BAC end sequencing; a centralized database for BAC fingerprints, hybridization and BAC end sequence data; training in BAC library construction and characterization through workshops at Clemson; and development of microarray technology for gene expression profiling and provision of this technology to the community. CUGI operates as a non-profit organization and will provide these services on a cost recovery basis. The service component of CUGI described here should be fully self-supporting by the end of the proposed funding period.