Antibody Facility: Generation and characterization of monoclonal antibodies directed against protein motifs involved in binding prosthetic groups

该项目的目的是生成、表征和提供特异性单克隆抗体，这是PROTRAIN大多数项目所需的。特别地，需要识别构象表位的抗体，并且因此可以在用辅基加载之前和之后区分蛋白质。详细地，该程序包括小鼠的免疫接种、血清滴度的监测、脾细胞的分离、与预培养的骨髓瘤细胞的融合、杂交瘤选择、通过ELISA和免疫印迹鉴定和表征分泌的抗体、生产性克隆的选择、培养和储存，以及最后鉴定抗体表位。具有所需基础设施的中央设施是经济的，并将允许将这些任务交给专家。因此，该项目要求招聘一名经验丰富的技术助理，该技术助理多年来在产生表位特异性抗体领域取得了非常成功的工作，并要求提供相应的耗材。

The aim of this project is the generation, characterisation and provision of specific monoclonal antibodies, as needed in the majority of the projects of PROTRAIN. In particular, antibodies are wanted that recognize conformational epitopes and thus can discriminate between proteins prior to and after loading with prosthetic groups. In detail, this program comprises immunization of mice, monitoring of serum titers, isolation of spleen cells, fusion with precultivated myeloma cells, hybridoma selection, identifcation and characterisation of secreted antibodies by ELISA and immunoblotting, selection, cultivation and storage of productive clones, and finally the identification of antibody epitopes. A central facility with the required infrastructure is economic and will allow to place these tasks into expert hands. Therefore, this project is asking for the position to employ an experienced technical assistant who has been working very successfully in the field of generating epitope-specific antibodies for many years, and for the respective means for consumables.