Extremely Halophilic Bacteria: A Remarkably Tractable System for Teaching Molecular Techniques

生物科学（61）本项目将一种极端嗜盐（即“嗜盐”）细菌的独特和可处理的生态学、遗传学、生理学和生物化学应用于初级分子技术教学实验室。它基于Johnsons概述的教学技术（Johnson， D. W. and R.T. Johnson, 1993，合作学习与大学教学通讯，3:6-9），Herreid （Herreid， C.F, 1998, BioScience 48:553-559）进一步详述。该项目实现了四个目标：1)使学生意识到作为分子技术实验室模型的一组生物生态学的重要性和相关性；2)学生了解到生物学是一门定量科学，而不仅仅是一门观察科学；3)所有学生平等参与真正的“动手”分子生物学实验室；4)学生将在高级高级课程和独立的本科研究项目中为更复杂的分子实验做好准备。学生和教师的专业和背景传统上不被认为是“分子”的本质是欢迎和鼓励报名参加这门课程。此外，正在制作和散发一份实验室手册（纸质版和网络版），并应要求提供菌株和环境样品。

Biological Science (61) This project adapts the unique and tractable ecology, genetics, physiology and biochemistry of an extremely halophilic (i.e., "salt loving") bacterium to a junior level teaching laboratory in molecular techniques. It is based on pedagogical techniques outlined by the Johnsons (Johnson, D. W. and R.T. Johnson, 1993, Cooperative Learning and College Teaching Newsletter, 3:6-9), further detailed by Herreid (Herreid, C.F., 1998, BioScience 48:553-559).This project accomplishes four objectives: 1) students are made aware of the importance and relevance of the ecology of the group of organisms used as models in the molecular techniques laboratory; 2) students learn that biology is a quantitative science, not solely an observational one; 3) all students participate equally in an authentic "hands-on" molecular biology laboratory; and 4) students are prepared for more sophisticated molecular experiments in an advanced senior level course and in independent, undergraduate research projects. Students and faculty whose majors and backgrounds are traditionally not considered "molecular" in nature are welcomed and encouraged to enroll in the course. In addition, a laboratory manual (both hard copy and Web-based) is being produced and disseminated, and bacterial strains and environmental samples are supplied for the asking.