Collaborative Research: Species Delimitation and Phylogenetic Relationships within Isoetes (Isoetaceae) Using Multiple Data Sets

9981460 Hoot Isobacterium Delimitation and Phylogenetic Relationships within Isobacterium using multiple Data setsIsobacterium是一个不开花植物属，在古代（古生代）广泛分布。 它们分布于世界各地，具有多样的生态适应性。 等离子体植物可以在溪流、湖泊和短暂的水池中找到。 该植物的形态非常简单，由窄而长的叶子组成，叶子在基部产生孢子，根茎肿胀。 这种简单的形式（形态学）提供了很少的特征，使得物种的鉴定和物种关系的确定变得困难。 此外，Isobacterium物种很容易相互杂交，进一步混淆了我们对物种界限和关系的理解。该项目有三个主要目标：1）提供一个全世界Isobacterium属的进化树（物种发生），2）识别和界定Isobacterium内的物种，3）识别杂交物种形成中涉及的原始亲本。 由于形态学提供的这一组的字符很少，额外的DNA序列形式的分子数据将被收集。 在过去的十年里，DNA序列数据一直是一个宝贵的工具，帮助科学家了解各种生物体的进化关系。 为了更好地了解全世界Isopropylene物种的关系，我们将使用两个DNA区域，一个位于叶绿体基因组（atpB/rbcL基因间间隔区），一个位于细胞核（内部转录间隔区- ITS）。 为了定义物种和确定杂交物种的起源，我们将使用一个参与植物繁殖的基因（LEAFY第二内含子）内的高度可变区。Hoot（Universityof Wisconsin-Milwaukee）和W.卡尔·泰勒（密尔沃基公共博物馆）。 胡特将主要负责分子工作，C。泰勒将参与植物收集（带他到遥远的地点在南美洲，非洲和其他大陆）和形态学研究的Isobacterium。

9981460HootSpecies Delimitation and Phylogenetic Relationships within Isoetes UsingMultiple Data SetsIsoetes are a genus of nonflowering plants which were widespread during ancient times (Paleozoic). They have a worldwide distribution and diverse ecological adaptations. Isoetes plants may be found in streams , lakes, and ephemeral pools. The form of the plant is very simple, consisting of narrow, elongated leaves which produce spores at their base and a swollen root stock. This simple form (morphology) provides few characters, making the identification of species and determination of species relationships difficult. In addition, Isoetes species readily hybridize with each other, further confusing our understanding of species limits and relationships.This project has three main objectives: 1) to provide an evolutionary tree(phylogeny) for the genus throughout the world, 2) to identify and delimitspecies within Isoetes, and 3) to identify the original parents involved inthe formation of hybrid species. Since morphology provides few characters for this group, additional molecular data in the form of DNA sequences will be collected. In the past ten years, DNA sequence data have been an invaluable tool, helping scientists understand evolutionary relationships in a wide varietyof organisms. To better understand relationships of Isoetes species worldwide, we will use two DNA regions, one located in the chloroplast genome (atpB/rbcL intergenic spacer region) and one in the nucleus (internal transcribed spacer regions - ITS). To define species and determine the origin of hybrid species, we will use a highly variable region within a gene involved in plant reproduction (LEAFY second intron).This work is a collaborative effort between Drs. Sara B. Hoot (Universityof Wisconsin-Milwaukee) and W. Carl Taylor (Milwaukee Public Museum). S.Hoot will be largely responsible for the molecular work, C. Taylor will beinvolved in plant collection (taking him to distant sites in South America,Africa and other continents) and morphological studies of Isoetes.