Temperature regulation of circadian clock proteins from Chlamydomonas and their relevance for entrainment by temperature cycles

除了光暗之外，温度循环是将生物钟同步到24小时周期的主要环境信号之一。为了控制温度周期的夹带，某些时钟组件应该能够响应生理范围内的温度变化。我们最近发现，莱茵衣藻（Chlamydomonas reinhardtii）的几个时钟相关蛋白可以整合温度信息，并且它们的温度依赖性调节是相互关联的。这些蛋白包括rna结合蛋白CHLAMY1的C1和C3亚基，酪蛋白激酶1 （CK1）和蛋白磷酸酶PP2A和/或PP5。它们在不同温度（18℃和28℃）下的表达、修饰和/或活性存在差异，差异在10℃，但都在衣藻的生理范围内。到目前为止，对其中一些成分的温度调节的改变导致了温度周期对生物钟同步的干扰。在本研究中，我们旨在进一步研究温度控制的分子钟网络。我们想要识别和功能表征一些负责的交易因子，在18ºC上调c3和28ºC上调ck1，并检查它们在温度循环中的作用。

Beside light-dark, temperature cycles are one of the main environmental cues that synchronize circadian clocks to a period of 24h. For control of entrainment by temperature cycles, certain clock components should be able to respond to changes of temperature within the physiological range. We have recently found that several clock-relevant proteins of the green alga Chlamydomonas reinhardtii can integrate temperature information and that their temperature-dependent regulation is interconnected. These proteins include the C1 and C3 subunits of the RNA-binding protein CHLAMY1, casein kinase1 (CK1) and protein phosphatases PP2A and/or PP5. They are differentially expressed, modified and/or active at different temperatures (18 and 28ºC) that differ in 10ºC, but are in the physiological range of Chlamydomonas. Alterations of the temperature regulation of some of these components that have been conducted so far resulted in disturbances of the synchronization of the circadian clock by temperature cycles. In this study, we aim to further examine the temperature-controlled molecular clock network. We want to identify and functionally characterize some of the responsible trans-acting factors, up-regulating c3 at 18ºC and ck1 at 28ºC and examine their role in entrainment by temperature cycles.