How the green alga Chlamydomonas reinhardtii keeps time
How the green alga Chlamydomonas reinhardtii keeps time
复制标题
绿藻莱茵衣藻如何计时
DOI：
10.1007/s00709-010-0113-0
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发表时间：
2010
期刊：
影响因子：
2.9
通讯作者：
Mittag
中科院分区：
文献类型：
--
作者：
Schulze;Prager;Kießling;Mittag
单细胞绿色藻类莱茵衣藻有两个鞭毛和一个原始的视觉系统，眼斑器，它允许细胞感光。大约40年前，人们发现生物钟控制着它的趋光运动。从那时起，一些昼夜节律，如趋化性，细胞分裂，紫外线敏感性，粘附玻璃，或淀粉代谢的特点。其全基因组序列的可用性沿着同源性研究和几个亚蛋白质组的分析renderC. reinhardtias是一种优秀的真核模式生物，可以研究其不同组织水平的昼夜节律钟。先前的研究指出了几种潜在的光感受器，它们可能参与转发光信息以携带其生物钟。然而，为此目的，实验数据仍然缺失。在过去的几年里，几个组件的功能特点是很可能是振荡机械的一部分。因为它们表达水平的改变或基因的插入诱变导致至少两个独立测量的节律的相位、周期或幅度的缺陷。这些包括几种叶绿体节律（ROC）蛋白，一种平行参与光周期控制的CONSTANS蛋白（CrCO），以及昼夜RNA结合蛋白CHLAMY 1的两个亚基。后者也与昼夜输出过程密切相关。几个候选者，包括大量的ROCs，CrCO和酪蛋白激酶1，其表达的改变影响昼夜节律钟，并行对子细胞的释放，鞭毛形成和/或运动产生严重影响，表明这些过程是相互关联的。莱因哈德氏菌未来具有挑战性的任务将是深入了解时钟网络，并找出与时钟相关的因素在功能上是如何连接的。在这方面，系统生物学的方法肯定会有助于在未来提高我们对C的理解。莱因哈德蒂克洛克机械公司。
The unicellular green algaChlamydomonas reinhardtiihas two flagella and a primitive visual system, the eyespot apparatus, which allows the cell to phototax. About 40 years ago, it was shown that the circadian clock controls its phototactic movement. Since then, several circadian rhythms such as chemotaxis, cell division, UV sensitivity, adherence to glass, or starch metabolism have been characterized. The availability of its entire genome sequence along with homology studies and the analysis of several sub-proteomes renderC. reinhardtiias an excellent eukaryotic model organism to study its circadian clock at different levels of organization. Previous studies point to several potential photoreceptors that may be involved in forwarding light information to entrain its clock. However, experimental data are still missing toward this end. In the past years, several components have been functionally characterized that are likely to be part of the oscillatory machinery ofC. reinhardtiisince alterations in their expression levels or insertional mutagenesis of the genes resulted in defects in phase, period, or amplitude of at least two independent measured rhythms. These include several RHYTHM OF CHLOROPLAST (ROC) proteins, a CONSTANS protein (CrCO) that is involved in parallel in photoperiodic control, as well as the two subunits of the circadian RNA-binding protein CHLAMY1. The latter is also tightly connected to circadian output processes. Several candidates including a significant number of ROCs, CrCO, and CASEIN KINASE1 whose alterations of expression affect the circadian clock have in parallel severe effects on the release of daughter cells, flagellar formation, and/or movement, indicating that these processes are interconnected inC. reinhardtii. The challenging task for the future will be to get insights into the clock network and to find out how the clock-related factors are functionally connected. In this respect, system biology approaches will certainly contribute in the future to improve our understanding of theC. reinhardtiiclock machinery.
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DOI：
10.1016/s0169-328x(99)00277-6
发表时间：
1999-12
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
M. Zylka;S. Reppert
通讯作者：
M. Zylka;S. Reppert
影响因子：
14.9
作者：
Tsuda K;Kuwasako K;Takahashi M;Someya T;Inoue M;Terada T;Kobayashi N;Shirouzu M;Kigawa T;Tanaka A;Sugano S;Güntert P;Muto Y;Yokoyama S
通讯作者：
Yokoyama S
昼夜节律 RNA 结合蛋白 CHLAMY1 的两个亚基都可以整合温度信息1[W]
影响因子：
7.4
作者：
Olga Voytsekh;S. Seitz;Dobromir Iliev;M. Mittag
通讯作者：
M. Mittag
影响因子：
2.9
作者：
E. Sato;I. Sagami;T. Uchida;Akira Sato;T. Kitagawa;J. Igarashi;Toru Shimizu
通讯作者：
E. Sato;I. Sagami;T. Uchida;Akira Sato;T. Kitagawa;J. Igarashi;Toru Shimizu
DOI：
--
发表时间：
2006
期刊：
影响因子：
--
作者：
Dobromir Iliev;Olga Voystekh;M. Mittag
通讯作者：
M. Mittag