Automated DNA Sequencer for Microbial Ecology Studies

0070139（用于微生物生态学研究的自动DNA测序仪）GundersonA LICOR 4200L2自动DNA测序仪将用于田纳西理工大学的微生物生态学研究。对分子微生物生态学感兴趣的教师和学生组成了一个跨学科的核心，来自生物系、水资源管理、利用和保护中心和土木工程系的代表参与了微生物生态学领域的资助研究。研究活动已经超出了我们的人工测序设备所能支持的水平。将立即使用自动测序仪的研究项目包括：基于rrna的切萨皮克湾寄生鞭毛虫分布调查、原生物食物液泡含量分析、白蚁鞭毛虫原核共生体的鉴定、军团菌样阿米巴原虫病原体存在的自然和人工栖息地筛选、农药对人工湿地原核生物群落影响的测定。虽然微生物生态学家将是这台机器的主要用户，但如果有的话，其他教员也会使用自动测序仪。自动DNA测序仪将促进TTU教师正在进行的研究，帮助为新的环境科学博士项目提供必要的基础设施支持，并允许研究生和本科生参与DNA测序项目。它的存在也将消除使用放射性同位素的风险和不便。

Abstract 0070139 (Automated DNA Sequencer for Microbial Ecology Studies) GundersonA LICOR 4200L2 automated DNA sequencer will be used for microbial ecology studies being conducted at TennesseeTechnological University. An interdisciplinary nucleus of faculty and students interested in molecular microbial ecology has developed here, with representatives from the Department of Biology, the Center for the Management, Utilization and Protection of Water Resources, and the Department of Civil Engineering engaged in funded research in the area of microbial ecology. The research activities have grown beyond a level which can be supported by our manual sequencing facilities.The research projects that would immediately employ an automated sequencer include an rRNA-based survey of the distribution of parasitic dinoflagellates in Chesapeake Bay, the analysis of protistan food vacuole contents, the determination of the identity of prokaryotic symbionts of termite flagellates, the screening of natural and man-made habitats for the presence ofLegionella-like amoebal pathogens, and the determination of the effects of pesticides on prokaryotic communities living in constructed wetlands. Although microbial ecologists would be the primary users of the machine, other faculty members would also use an automated sequencer if one were available.An automated DNA sequencer would facilitate the ongoing research of faculty members at TTU, help provide necessary infrastructural support to a new Ph.D. program in environmental sciences, and allow both graduate and undergraduate students to be involved in DNA sequencing projects. It's presence would also eliminate the risk and inconvenience of radioisotope use.