An Automated DNA Sequencer for the Department of Biological Sciences, University of Alabama

获奖摘要- DBI-0070351“亚拉巴马大学生物科学系的自动DNA测序仪“积累基因组数据提供了一个机会来研究各种水生物种之间的进化关系。 蜗牛，双壳类，太阳鱼，鲈鱼和动孢子菌的系统发育研究提供了丰富的信息，生态学，地理学，以及这些不同群体的形态和生活史特征的演变。自动DNA测序仪将用于更有效和准确地对脊椎动物、无脊椎动物和微生物进行基因组研究。 使用的特定设备是LI-COR 4200 S-2基因ReadIR DNA分析系统。 LI-COR系统通过分离标记的DNA并自动扫描凝胶在两个红外（IR）波长处的荧光来产生高质量的原始图像数据。 LI-COR产生高精度读数，无需放射性标记。 除了DNA测序，LI-COR系统还具有微卫星DNA研究的硬件和软件功能。 除了提高研究生产力和产出外，该设备还将为对生物技术和基因组研究感兴趣的学生提供极好的培训机会。 目前，几乎所有的DNA测序都是用自动测序仪进行的。 拥有生物系的设备将提供宝贵的实践培训，并提高亚拉巴马大学生物学研究生和本科生的竞争力和知识基础。

Award abstract - DBI-0070351"An Automated DNA Sequencer for the Department of Biological Sciences, The University of Alabama"Accumulating genomic data provides an opportunity to examine evolutionary relationships within a wide variety of aquatic species. On going phylogenetic studies of snails, bivalves, sunfishes, basses, and zoosporic fungi provide a wealth of information on the ecology, biogeography, and evolution of morphological and life-history features of these diverse groups. An automated DNA sequencer will be used to conduct genomic studies more efficiently and accurately on vertebrates, invertebrates, and microorganisms. The specific equipment to be used is the LI-COR 4200S-2 Gene ReadIR DNA Analysis System. The LI-COR system produces high quality raw image data by electrophoretically separating labeled DNA and automatically scanning gels for fluorescence at two infrared (IR) wavelengths. LI-COR produces high accuracy reads and eliminates the need for radioactive labeling. In addition to DNA sequencing, the LI-COR system has hardware and software capabilities for microsatellite DNA research. In addition to increasing research productivity and output, this equipment will provide excellent training opportunities for students with an interest in biotechnology and genomic research. Virtually all DNA sequencing is conducted today with an automated sequencer. Having the equipment in the Biology Department will provide valuable hands-on training and increase the competitiveness and knowledge base of graduate and undergraduate biology students at the University of Alabama.