Characterization of Viruses on Patterned Cells

PI的目标是开发一种高通量筛选方法，以有效地测量和表征细胞培养物中病毒的生长。他计划评估他的方法的可行性。他的方法是基于这样的想法：（1）局限于生长成宿主细胞线性阵列的病毒将以特定病毒-宿主对的有用特征的速度传播;（2）在这种传播感染中，病毒和宿主成分的空间分布可以反映感染过程的特征阶段。更具体的目标是：（1）评估图案化宿主细胞如何影响它们在培养物中的生存力，（2）测量特定早期和晚期组分的标记物的空间分布，（3）测试是否可以使用感染到图案化细胞中的局部起始来增加单个样品产生的信息量，以及（4）评估宿主细胞的图案化如何影响病毒生长。

PI's objective is to develop a high-throughput screening method to efficiently measure and characterize the growth of viruses in cell culture. He plans to evaluate the feasibility of his approach. His approach is based on the idea that (1) viruses confined to grow into linear arrays of host cells will spread at velocities that are useful traits of the specific virus-host pair and (2) the spatial distribution of virus and host components in such propagating infections can reflect characteristic stages of the infection process. More specific aims are to: (1) assess how patterning host-cells affects their viability in culture, (2) measure the spatial distribution of markers for specific early and late components, (3) test whether localized initiations of infection into patterned cells can be used to increase the amount of information that a single sample yields, and (4) evaluate how the patterning of host cells influences viral growth.