A Genetic Analyzer for DNA Sequencing and DNA Fragment Analysis in the Basic Sciences at Georgetown University

该奖项提供资金帮助购买自动DNA测序仪以及样品制备和数据分析所需的相关设备和软件。该仪器在研究和培训工作中的使用将由一大群教师、学生和博士后共享。研究领域包括各种原生动物的表面蛋白调控、热带树木的基因流动、酵母基因调控和花色变异。现在生物学中的许多研究问题都需要使用基因型知识作为观察的基本单位。这些问题跨越不同的领域，包括与蛋白质结构和功能、基因调控、基因组组织、物种内和物种间关系、遗传多态性的时空组织以及种群或个体之间配子交换有关的假设。DNA测序和片段基因分型的自动化方法比它们所取代的人工方法更快、更准确，从而提高了数据的数量和质量。除了用于研究之外，教师们还计划在本科生和研究生课程中使用该仪器进行实际操作的实验练习，这在技术上已经超出了他们的实验室能力。

This award provides funds to aid in purchase of an automated DNA sequencer and related equipment and software needed for sample preparation and data analysis. Use of the instrumentation in research and training efforts will be shared by a large group of faculty, students and postdocs. Research areas to be addressed include regulation of surface proteins in various protozoa, gene-flow in tropical trees, yeast gene regulation and variability in flower color. Many research questions in biology now require use knowledge of genotypes as a basic unit of observation. These questions span diverse fields and include hypotheses related to protein structure and function, gene regulation, genome organization, relationships within and among species, spatial and temporal organization of genetic polymorphism and the exchange of gametes among populations or individuals. Automated approaches to DNA sequencing and fragment genotyping are faster and more accurate than the manual methods they replace, increasing the quantity and quality of data. In addition to use in research, the faculty plan to employ the instrument in hands-on laboratory exercises, now technically beyond their laboratory capabilities, for both undergraduate and graduate courses.