Postdoctoral Research Fellowship in Microbial Biology for FY2001

这一行动为2001财年NSF微生物生物学博士后奖学金提供了资金。该奖学金支持关于原生动物、微藻、真菌、古生物、细菌和病毒物种的基本生物学的培训和研究，这些物种通常不被认为是模式生物。此外，它还为最近的博士获得者提供了获得微生物生物学方面的额外培训的机会，在知名科学家的赞助下获得研究经验，并在本科生和研究生培训期间拓宽其科学视野，超越研究经验。这些奖学金还旨在帮助新科学家引导他们的研究工作跨越传统的学科界限，并利用独特的研究资源、地点和设施，包括外国地点。这项研究和培训计划的标题是“苯甲酸盐降解反硝化细菌和环境样本中的遗传多样性”。从受污染的垃圾填埋场河口沉积物中分离的细菌纯培养物，研究了在反硝化条件下能够降解苯甲酸盐的细菌的16S rRNA、亚硝酸还原酶和苯甲酰辅酶A还原酶的遗传多样性。此外，将直接从相同的沉积物中提取DNA，并将构建环境DNA克隆文库(元基因组文库)，以连接从16S rRNA基因获得的不同类型的遗传信息和参与环境中苯甲酸盐降解和反硝化的功能基因。

This action funds an NSF Postdoctoral Fellowship in Microbial Biology for FY2001. The fellowship supports training and research on the basic biology of protozoan, microalgal, fungal, archaeal, bacterial and viral species that are not generally considered to be model organisms. Further, it provides opportunities for a recent doctoral recipients to obtain additional training in microbial biology, to gain research experience under the sponsorship of established scientists, and to broaden his/her scientific horizons beyond the research experiences during the undergraduate and graduate training. These fellowships are further designed to assist new scientists to direct their research efforts across traditional disciplinary lines and to avail themselves of unique research resources, sites, and facilities, including foreign locations. The research and training plan is entitled "Genetic Diversity in Benzoate Degrading Denitrifying Bacteria and Environmental Samples." Genetic diversity of 16S rRNA, nitrite reductase, and benzoyl-CoA reductase in bacteria capable of degrading benzoate under denitrifying conditions are being investigated with bacterial pure cultures isolated from estuarine sediments of a contaminated landfill site. In addition, DNAs will be extracted directly from the same sediment and environmental DNA clonal libraries (metagenome library) will be constructed to link the different kind of genetic information obtained from 16S rRNA genes and functional genes involved in benzoate degradation and denitrification in the environment.