Biogeochemical Cycles of Carbon and Nitrogen in an Ice-Covered Volcanic Crater Lake

冰覆盖火山口湖碳氮生物地球化学循环项目描述这是一项为期1年的研究计划和探索性提案，旨在调查冰岛Grimsvotn活火山冰覆盖火山口湖的微生物生态学。该提案预计在Grimsvotn冰湖下有一个活跃的微生物群落。由于生物地球化学环境简单，与火山和冰川以外的其他输入隔离，因此被认为是进行耦合生物复杂性研究的绝佳地点。该提案将通过使用热水钻探设备在冰面上取样来评估四种假设。研究人员将从不同的湖泊深度采集样本，并使用显微镜、培养、稳定同位素分析、分子生物学工具、碳和氮固定试验以及与氮固定有关的基因的系统发育分析等方法进行检测。

0120435Biogeochemical Cycles of Carbon and Nitrogen in an Ice-Covered Volcanic Crater LakeProject DescriptionThis is a 1 year research planning and exploratory proposal to investigate the microbial ecology of and ice-covered crater lake within the active Grimsvotn Volcano in Iceland. The proposal anticpates that there is an active microbial community under the Grimsvotn ice-covered lake. Because of the simplicity of the biogeochemical environment and its isolation from other inputs other than volcanic and glacial, it is suggested to be an excellent location to pursue coupled biocomplexity studies. The proposal will evaluate four hypotheses by sampling the lake through the ice using hot-water drilling equipment. Samples will be collected from different lake depths and examined using: microscopy, cultivation, stable isotopic analyses, molecular biological tools, C and N fixation assays, and phylogentic analysis of genes involved in N fixation.