Quantitative Biochemistry Laboratory Experiments: Introduction of Isothermal Titration Calorimetry and Real Time PCR in the Undergraduate Curriculum

化学（12）州立大学纽约学院的化学系正在通过购买等温滴定量热仪（ITC）和实时PCR热循环仪/序列检测器来更新其生物化学、无机化学、物理化学和分析化学实验室课程。动机源于我们的强烈愿望，以扩大我们的学生的经验与现代生物化学仪器，增加探究和发现为基础的实验室实验主动学习，并提高参与本科研究项目，我们认为是化学或生物化学教育的一个组成部分。我们的最终目标是培养对科学感兴趣的学生，他们拥有必要的技能和解决问题的能力，能够及时解决重要问题。利用ITC和实时PCR热循环仪/序列检测器的一些基于发现的实验正在从研究和/或教育文献中改编。利用实时PCR热循环仪的实验正在生物化学实验室进行。ITC实验正在生物化学、无机和物理/分析实验室进行。例如，由威廉姆斯学院的Lawrence Kaplan教授（NSF DUE 9850820）和纽约汉密尔顿学院的Timothy Elgren教授（NSF-DUE 9951375）开发的关于分子相互作用研究的ITC实验正在我们的生物化学实验室中实施。Kaplan教授和Elgren教授都与我们分享了他们的课程，并同意作为我们基于探究的实验室的测试地点。我们相信，使用这些仪器的实验包括显着提高我们的学生在Geneseo的教育。

Chemistry (12)The Department of Chemistry at the State University of New York College at Geneseo is modernizing its Biochemistry, Inorganic, Physical, and Analytical Chemistry laboratory curriculum with the purchase of an Isothermal Titration Calorimeter (ITC) and a Real-Time PCR Thermalcycler/Sequence Detector. The motivation stems from our strong desire to broaden our students' experience with modern biochemistry instrumentation, increase inquiry- and discovery-based laboratory experiments for active learning, and to improve participation in undergraduate research projects that we believe are an integral part of a chemistry or biochemistry education. Our ultimate goal is to produce students who are excited about science and who possess the necessary skills and problem-solving abilities to solve timely and important problems.A number of discovery-based experiments utilizing the ITC and the Real-Time PCR Thermalcycler/Sequence Detector are being adapted from the research and/or education literature. Experiments utilizing the Real-Time PCR Thermalcycler are being carried out in the biochemistry laboratory. ITC experiments are being done in the Biochemistry, Inorganic and Physical/Analytical Laboratories. For example, ITC experiments on the study of molecular interactions, developed by Professor Lawrence Kaplan at Williams College (NSF DUE 9850820) and Professor Timothy Elgren at Hamilton College, NY (NSF-DUE 9951375), are being implemented into our biochemistry laboratory. Both Professor Kaplan and Professor Elgren have kindly shared their programs with us and have agreed to serve as test sites for our inquiry-based laboratories. We believe that inclusion of experiments using these instruments are significantly enhancing the education of our students at Geneseo.