Acquisition of a DNA Sequencer for Use in Undergraduate Biochemistry/Molecular Biology and DNA Computing Research and Teaching

获取用于本科生物化学/分子生物学和 DNA 计算研究和教学的 DNA 测序仪

• 批准号: 0420600
• 负责人: Wendy Pogozelski
• 金额: $ 10.87万
• 依托单位: SUNY College at Geneseo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0420600&HistoricalAwards=false
• 关键词: Acquisition; DNA; Sequencer; Use; Undergraduate

A grant has been awarded to the State University of New York (SUNY) College at Geneseo under the direction of Dr. Wendy Pogozelski for acquisition of a DNA sequencer. The sequencer will be used to expand the number and scope of undergraduate projects offered in DNA research and to support new curriculum initiatives in DNA sequencing, DNA computing, and bioinformatics. The research activities, designed for undergraduate involvement, include the identification of regions of human mitochondrial DNA that are subject to deletions when treated with gamma rays; comparison of the mitochondrial DNA codes of various strains of mice; studying the genetic relationships cannibalistic embryos in a brood of the Antarctic sea star; identifying microbes for bioremediation in the Kyzil Kum desert of Uzbekistan, and determining DNA sequences of long DNA strands that represent patterns or codes in DNA computing problems. In addition, the sequencer is be used for verification of DNA sequences and rRNA sequences in the Biochemistry Laboratory class and Microbiology Laboratory classes at Geneseo. These projects are of use to society in that they represent efforts in developing new tools for biodosimetry (ways to measure accidental radiation damage in humans), preventing depletion of Antarctic marine life, partnering with the international scientific community for environmental bioremediation and stabilization, developing new methods for computing in an effort to solve computationally hard problems, and developing good models for mitochondrial DNA diseases. In addition, they are vital tools in educating undergraduates in the DNA sciences and preparing them for careers in scientific research.

在Wendy Pogozelski博士的指导下，位于Geneseo的纽约州立大学(SUNY)学院获得了一笔赠款，用于购买DNA测序仪。该测序仪将用于扩大DNA研究提供的本科项目的数量和范围，并支持DNA测序、DNA计算和生物信息学的新课程倡议。这些研究活动是为本科生设计的，包括确定人类线粒体DNA在受到伽马射线处理时可能缺失的区域；比较不同品系的小鼠的线粒体DNA密码；研究南极海星幼崽中食人胚胎的遗传关系；识别乌兹别克斯坦Kyzil Kum沙漠中用于生物修复的微生物，以及确定代表DNA计算问题中模式或密码的长DNA链的DNA序列。此外，该测序仪还可用于Geneseo生物化学实验室和微生物实验室的DNA序列和rRNA序列的验证。这些项目对社会是有用的，因为它们代表了以下方面的努力：开发新的生物剂量测定工具(测量人类意外辐射损害的方法)、防止南极海洋生物的枯竭、与国际科学界合作进行环境生物修复和稳定、开发新的计算方法以努力解决计算困难的问题，以及开发线粒体DNA疾病的良好模型。此外，它们也是培养DNA科学本科生并为他们从事科学研究做好准备的重要工具。