CAREER: Coordinating Primary and Secondary Metabolic Activities for Enhancing Terpenoid Indole Alkaloid Production from Catharanthus roseus Cultures

0134511Lee该研究的目的是开发技术，以显着增加萜类吲哚生物碱（TIA）的生产从细胞培养的C。罗斯 所提出的假设是，为了最大限度地生产诸如TIA等次级代谢物，必须平衡各级前体和能源的相对流动。 为实现这一平衡，增加军备透明和透明度，建议的具体目标是：（1）增加初级代谢和次级代谢的通量，（2）最大化TIA产生的代谢通量，同时维持细胞机制的适当代谢通量以支持必需的细胞活性，（3）调节糖酵解、柠檬酸和磷酸戊糖途径之间的通量以最大化次级代谢产物产生，以及（4）表征各种途径和活动的最佳平衡。 该研究可以提高重要的抗肿瘤和抗高血压化合物的产率。

0134511LeeThe objective of the proposed research is to develop techniques to significantly increase terpenoid indole alkaloid (TIA) production from cell cultures of C. roseus. The proposed hypothesis is that to maximize the production of secondary metabolites such as TIAs, the relative flows of precursors and energy resources at various levels must be balanced. The proposed specific aims for achieving this balance for increasing TIA production are to: (1) increase flux to both primary and secondary metabolism, (2) maximize the metabolic flux to TIA production while maintaining an appropriate metabolic flux to cellular machinery for supporting essential cellular activities, (3) tune the flux between the glycolysis, citric acid, and pentose phosphate pathways for maximum secondary metabolite production, and (4) characterize the optimum balance the various pathways and activities. This research could produce results to increase the yield of important antitumor and antihypertensive compounds.