Examining the mechanism of the gene simplet (smp) in nerve-dependent appendage regeneration of the zebrafish

为了鉴定器官再生启动过程中激活的基因，我们对再生的心脏、肝脏和鳍组织进行了微阵列分析。我们从这些再生组织的微阵列图谱中鉴定出了simplet（smp）基因，我们发现它是再生生长过程中细胞增殖所必需的。我们还发现，smp的敲低使音刺猬（shh）的表达失调，shh是一种再生生长和调节祖细胞群所需的形态原。关于该基因的唯一的其他出版物表明smp也是早期发育过程中细胞增殖和形成模式所必需的。关于Smp调节细胞增殖和shh转录的分子机制知之甚少。由于细胞增殖和调节SHH是组织发育和再生的组成部分，我们打算确定如何SMP功能的SMP蛋白的结构功能分析，通过确定相互作用的合作伙伴，通过测试他们参与SMP活性，通过确定SMP是如何调节，并通过评估它是否参与神经依赖的附件再生的信号转导。

To identify genes activated during the initiation of organ regeneration, we performed microarray profiling of regenerating heart, liver, and fin tissues. We identified the gene simplet (smp) from our microarray profiles of these regenerating tissues, and we found that it is required for cell proliferation during regenerative outgrowth. We also found that knockdown of smp deregulated the expression of sonic hedgehog (shh), a morphogen required for regenerative outgrowth and for regulation of progenitor cell populations. The only other publication of the gene shows that smp is also required for cell proliferation and patterning during early development. Little is known about the molecular mechanism(s) through which Smp regulates cell proliferation and shh transcription. Because cell proliferation and the regulation of shh are integral components of tissue development and regeneration, we intend to determine how smp functions by structure function analysis of the Smp protein, by identifying interaction partners, by testing their involvement in smp activity, by determining how smp is regulated, and by assessing whether it is involved in signaling transduction of nerve-dependent appendage regeneration.