MRI/RUI: Acquisition of Instrumentation for High Resolution Light Microscopy and Image Processing at Mount Holyoke College

申请编号6113720PI: Sue Ellen F. Gruber为Mount Holyoke学院购置高分辨率光学显微镜和图像处理仪器摘要一项资助已授予Mount Holyoke学院的Sue Ellen F. Gruber博士和其他五位生物学家，用于购置高分辨率光学显微镜和图像处理仪器。光学显微镜是这些教师及其生物系本科生研究的各种生物问题分析的核心。将获得的仪器，包括一个研究质量的荧光显微镜，加上一个高分辨率冷却CCD照相机和计算机硬件和软件，用于图像处理，将增加这些研究人员的研究需要，在每种情况下都将允许现有设备无法进行的实验和分析。与此同时，这笔拨款将加强生物系学生的研究训练，以及生物化学和神经科学与行为两个相关专业的学生。新的仪器，将成为一个综合的显微镜设施的一部分，将补充现有的电子显微镜与最新的先进的几种模式的光学显微镜。六位参与研究的生物学教员所调查的项目范围很广。其中一项研究的是母亲和胎儿之间影响怀孕成功的免疫相互作用。第二篇探讨了抗疟疾药物在微生物中的作用。第三个项目是北美独一无二的，旨在记录被忽视的无脊椎动物群体的生物多样性。其余的项目剖析导致植物程序性细胞死亡的事件，整理可能导致小鼠艾滋病相关免疫缺陷的细胞表面特征的特性，并检查类固醇激素在果蝇发育中的作用。在所有情况下，获得和处理高质量显微镜图像的能力的增强将开辟新的实验途径。芒特霍利奥克学院是一所致力于培养女性成为生产社会各个方面的领导者的机构，在培养女科学家方面有着特别杰出的记录。它的使命是通过与强调用现代设备进行实践研究的有成就的教师合作，培养学生像实践科学家一样思考和行动。因此，新仪器的好处将超越特定的教师研究成果，因为它们培养了一代未来科学家的研究培训。

Proposal 6113720PI: Sue Ellen F. GruberAcquisition of Instrumentation for High Resolution Light Microscopy and Image Processing at Mount Holyoke CollegeABSTRACTA grant has been awarded to Dr. Sue Ellen F. Gruber and five other biologists at Mount Holyoke College to acquire instrumentation for high resolution light microscopy and image processing. Light microscopy is core to the analysis of diverse biological questions under investigation by these faculty and their undergraduates in the Biology Department. The instruments to be obtained, including a research-quality fluorescence microscope coupled to a high resolution cooled CCD camera and computer hardware and software appropriate for image processing, will augment the research needs of these researchers, and in each case will permit experimentation and analysis not possible with existing equipment. This grant will, at the same time, enhance the research training of students in the Biology Department, as well as in the two allied programs of Biochemistry, and Neuroscience and Behavior. The new instrumentation, to become part of an integrated microscopy facility, will complement the existing electron microscopes with up-to-date sophistication in the several modes of light microscopy.Projects under investigation by the six participating biology faculty members are wide-ranging. One examines the immunological interactions between a mother and fetus that influence the success of pregnancy. A second explores the action of antimalarial drugs in microorganisms. A third, unique in North America, aims to document the biodiversity of neglected invertebrate groups. The remaining projects dissect the events giving rise to programmed cell death in plants, sort out the identity of cell surface features that may be responsible for AIDS-related immunodeficiency in mice, and examine the role of steroid hormones in the development of fruitflies. In all instances, the enhanced ability to obtain and process high quality microscope images will open up new avenues of experimentation.Mount Holyoke College is an institution dedicated to preparing women to be leaders in every part of productive society and has an especially outstanding record in training women scientists. Its mission is to train students to think and act as practicing scientists, by working with accomplished faculty who emphasize hands-on research with modern equipment. Thus, the benefits of the new instrumentation will extend beyond the particular faculty research accomplishments, as they nurture the research training of a generation of future scientists.