SGER: Interactions between Microbial Nitrogen Cycling and Acid Mine Drainage Contaminants in Impacted Streams

[0221880 Silverstein]酸性矿井排水对天然生物地球化学氮循环的影响将通过在接收水中发现的较低pH下微生物氮和铁转化的相互作用来研究。本文将研究受酸性主排水影响的河流中铁离子和硝酸盐作为电子受体的竞争、铁离子作为电子供体的反硝化作用以及细菌还原对铁离子和其他重金属的动员作用。受影响和未受影响溪流中的物种鉴定将利用DNA探针和FISH技术进行，显微计数将用于对趋化石养铁氧化剂和异养嗜酸菌进行定量估计。

0221880 Silverstein The effects of acid mine drainage on natural biogeochemical nitrogen cycling will be studied with respect to the interaction of microbial nitrogen and iron transformations at the lowered pH found in receiving waters. Competition between ferric iron and nitrate as electron acceptors, use of ferrous iron as electron donor in denitrification, and mobilization of iron and other heavy metals from bacterial reduction will be investigated in acid main drainage impacted streams. Species identification in impacted and unimpacted streams will be made utilizing DNA probes and the FISH technique and microscopic counts will be used to produce quantitative estimates of chemolithotrophic iron oxidizers and heterotrophic acidophiles.