FIBR Planning: A Systems Approach to Study Redox Regulation of Functions of Photosynthetic Organisms

氧化还原化学是光合生物如蓝藻和植物的核心功能。在这个项目中，研究将开始对细胞氧化还原状态对蓝藻的整体功能的影响进行系统分析，并在未来应用于植物。重点是四个蓝藻物种，每个都有一个完整的测序基因组。两个物种，集胞藻6803和鱼腥藻7120，适合高通量基因组水平的操作。虽然库存的基因，转录本和蛋白质可用于这些生物体，他们是不足以理解这些蓝藻的复杂功能的组织层次结构。该项目将采用多学科方法。项目团队的专业知识涵盖分子生物学，生物化学，生物信息学和工程系统方法，包括非线性建模和控制，估计和统计分析。该团队的实验研究经验涵盖了蓝藻、非维管植物（苔藓）和维管植物（拟南芥）的模型系统。多个网络模型将研究蓝藻细胞分子组分之间的相互作用，制定工作假设，来自这些假设的数据将完善模型。这一过程将产生对这些生物体中氧化还原控制网络的组织和功能的基本见解。这种在蓝藻中模拟网络并将其应用于植物的方法将突出陆地植物进化过程的性质。在规划期间，（a）团队将参加每月会议;（B）将组织一次研讨会;（c）将制定一个完整的FIBR项目计划。本科生、研究生和博士后学员将参加这些活动。

Redox chemistry is central to the functions of oxygenic photosynthetic organisms such as cyanobacteria and plants. In this project, research will be initiated on a SYSTEMS analysis of the impact of cellular redox status on the overall function of cyanobacteria, with future applications to plants. The focus is on four cyanobacterial species, each with a completed sequenced genome. Two species, Synechocystis 6803 and Anabaena 7120, are amenable to high-throughput genome level manipulations. Although the inventory of genes, transcripts and proteins are available for these organisms, they are inadequate to comprehend the organizational hierarchy of the complex functions of these cyanobacteria. The project will involve a multidisciplinary approach. Expertise of the project team spans molecular biology, biochemistry, bioinformatics and systems approach in engineering, including nonlinear modeling and control, estimation and statistical analysis. Experimental research experience of the team spans model systems in cyanobacteria, non-vascular (moss) and vascular plants (Arabidopsis). Multiple network models will study interactions between molecular components of cyanobacterial cells, formulate working hypotheses, and data from such hypotheses will refine the model. This process will generate fundamental insights into the organization and function of the redox control network in these organisms. This approach to model a network in cyanobacteria, then to apply it to plants, will highlight the nature of processes during the evolution of land plants.During this planning period, (a) the team will participate in monthly meetings; (b) a workshop will be organized; and (c) the plan for a full-length FIBR project will be formulated. Undergraduate, graduate and postdoctoral trainees will participate in these activities.