An Inquiry-based Cell Biology Laboratory in which Student Research Teams Investigate the Heat Schock Response Using a Cell Culture Model

生物科学（61）这个项目是建立一个基于调查的本科细胞生物学实验室在斯通希尔学院陪同所需的讲座课程。实验室课程是仿照两个以前资助的CCLI项目（0088185，9952672）。通过团队项目进行探究式学习的主题改编自第一个项目（0088085），热休克反应的统一主题是我们对第二个项目（9952672）中绿色荧光蛋白的类似主题的实施。两个模块中的第一个模块指导学生基本的细胞和分子生物学技术，而第二个模块允许学生通过合作研究团队的工作来展示他们的技术能力，以研究实验条件对CHO细胞中热休克蛋白表达的影响。学生的结果是在科学文章，海报，并在一个小型研讨会上向学院社区开放的口头报告的风格写的实验室报告的形式进行沟通，每个同行和教师评审。该项目的目标是：（1）建立一个以开放式发现为基础的研究科学问题的实验室;（2）发展和提高关键的专业技能：假设和协议的制定，使用适当控制的实验，收集，分析和解释数据，并形成结论;（3）培养合作学习的氛围;及（4）发展及提高学生的分析及口头及书面沟通技巧。建议活动的智力优点：细胞生物学实验室向学生介绍基因组学及蛋白质组学的基础知识，这两个高度技术性及具挑战性的领域将彻底改变我们对生命过程的理解。因此，生物化学和生物学专业的本科生获得这些领域的研究技术及其应用的实践知识和实践经验是至关重要的。拟议活动产生的更广泛的影响：完成细胞生物学课程和实验室的斯通希尔本科生将展示增强的研究技能。被选中的学生将担任导师，问题解决者和榜样，为学业有天赋，经济上处于不利地位的高中生进行全职研究在石山作为ACS的项目种子的一部分（夏季教育经验的dispenser）。

Biological Sciences (61) This project is establishing an inquiry-based undergraduate Cell Biology Laboratory at Stonehill College to accompany the required lecture course. The laboratory course is modeled on two previously funded CCLI projects (0088185, 9952672). The theme of inquiry-based learning through team projects is adapted from the first project (0088085), and the unifying theme of heat shock response is our implementation of a similar theme on green fluorescent protein from the second project (9952672). The first of two modules instructs students in fundamental cell and molecular biology techniques, while the second allows the students to demonstrate their competencies with the techniques by working in collaborative research teams to investigate the effects of an experimental condition on the expression of heat shock proteins in CHO cells. Student results are communicated in the form of lab reports written in the style of a scientific article, a poster, and an oral presentation at a mini-symposium open to the College community, and each is peer- and faculty-reviewed. The objectives of the proposed project are (1) to establish a laboratory with an open-ended discovery-based approach to investigate a scientific question; (2) to develop and improve crucial professional skills: hypotheses and protocol development, experimentation using proper controls, collection, analysis, and interpretation of data, and formation of conclusions; (3) to foster an atmosphere of cooperative learning; and (4) to develop and improve students' analytical and oral and written communication skills.Intellectual Merit of the Proposed Activity: The Cell Biology Laboratory introduces students to the basics of genomics and proteonomics, two highly technical and challenging fields that will revolutionize our understanding of life processes. Therefore, it is essential that undergraduate Biochemistry and Biology majors obtain practical knowledge and hands-on experiences with the research techniques of these fields and their applications.Broader Impacts Resulting from the Proposed Activity: Stonehill undergraduates who complete the Cell Biology course and lab will demonstrate strengthened research skills. Selected students will serve as mentors, problem-solvers, and role models for academically-gifted, economically-disadvantaged high school students conducting fulltime research at Stonehill as part of the ACS's Project SEED (Summer Educational Experience for the Disadvantaged).