Adaptive Mutations: A Consequence of Prokaryotic Differentiation

拉斯维加斯内华达州大学的罗纳德亚斯宾博士获得了一项奖励，以研究细胞分化过程与提高革兰氏阳性细菌枯草芽孢杆菌突变率的遗传机制之间的相互作用。 新达尔文主义理论在很大程度上依赖于随机突变过程的能力，以提供进化过程继续所需的大量遗传多样性。然而，从数学模型和轶事观察中可以清楚地看到，完全随机的诱变不可能在可用的时间内提供所有必要的事件，使我们从一些非常简单的生命形式发展到人眼的复杂性。因此，在孟德尔遗传学的合理范围内解释必要的遗传多样性存在问题。在过去的几十年里，已经观察到许多多样性产生机制的例子，这些机制是自然遗传过程的重要组成部分。DNA已被证明是移动的，细胞已被证明能够提高其突变率，以应对环境的挑战。当细胞复制或复制其遗传物质时，以双链DNA的形式，已经证明新遗传物质中发生突变的速率从一条链到另一条链并不恒定。此外，有人认为，将DNA中所含的遗传信息翻译成蛋白质的过程也会显著影响突变率和所产生突变的位置。该项目探索了其他遗传机制，以提高突变率，并可能影响突变发生的位置。因此，三个充分表征和完全测序的基因的B。枯草芽孢杆菌被用于研究在环境压力期间产生突变的机制。目的是确定这些突变是否在已经分化的细菌亚群中产生，以便在培养物中产生必要的遗传多样性。对突变频率和由此产生的菌落的分析正在进行数学建模。因此，该项目代表了分子生物学家/遗传学家和统计学家之间的积极合作，并包含非常重要的教育组成部分。

An award is being made to Dr. Ronald Yasbin of the University of Nevada Las Vegas to study the interaction between processes of cellular differentiation and genetic mechanisms for enhancing mutation rates in the Gram Positive bacterium, Bacillus subtilis. Neo-Darwinian theory has counted heavily upon the ability of the random mutagenesis processes to provide a considerable amount of the genetic diversity necessary for the evolutionary process to continue. However, it is clear from both mathematical modeling as well as just anecdotal observations that completely random mutagenesis could not have provided all of the necessary events to have gotten us from some very simple form of life to the complexity of the human eye in the amount of time that has been available. The problem therefore exists as to explain the necessary genetic diversity within the legitimate confines of Mendelian genetics.During the last few decades numerous examples of diversity generating mechanisms, that are important parts of the natural genetic process, have been observed. DNA has been shown to be mobile and cells have been demonstrated to be capable of enhancing their mutation rates in response to environmental challenges. As cells replicate or duplicate their genetic material, in the form of double stranded DNA, is has been demonstrated that the rate at which mutations occur in the new genetic material is not constant from one strand to other. Furthermore, it has been suggested that the processes that exist to translate the genetic information contained in the DNA into protein can also significantly effect mutation rates and the location of generated mutations. This project explores additional genetic mechanisms for enhancing mutation rates and for potentially influencing in where the mutations will occur. Accordingly, three well characterized and fully sequenced genes of the B. subtilis are being used to investigate the mechanisms that generate mutations during periods of environmental stress. The goal is to determine whether or not these mutations are being generated in a subpopulation of the bacteria that have been differentiated in order to generate essential genetic diversity within the culture. The analysis of the mutation frequencies and the resultant colonies are being subjected to mathematical modeling. Accordingly, this project represents an active collaboration between molecular biologists/geneticists and statisticians and contains a very important educational component.