Regulation of plant metabolism in response to low oxygen concentrations

在本提案中，我们的目标是表征参与植物对氧浓度适度下降的主动反应的信号成分，因为它发生在生理条件下的组织内。为了做到这一点，我们将主要关注研究导致基因表达重编程的信号通路，以应对低氧。在过去的几年里，我们已经发现了大量的缺氧诱导的代谢和分子反应。现在，我们想要研究参与调节各种反应的传感和信号级联。我们将使用RT-PCR转录因子分析来鉴定参与调节基因表达和适应低氧的氧相关转录因子。选定的转录因子将在体内和体外进行表征，以揭示它们的生理功能和它们的遗传相互作用，以触发氧敏感启动子的转录。目标序列将在通过低氧反应的QTL分析确定的染色体切片中进行探索。荧光素酶报告筛选用于发现氧信号突变缺陷的基因，将基因表达的变化与低氧联系起来。

In this proposal we aim to characterize signalling components that are involved in the proactive response of plants to a moderate decrease in the oxygen concentration as it occurs under physiological conditions within their tissues. To do this, we will mainly focus on investigating signalling pathways that lead to reprogramming of gene expression in response to low oxygen. In the past years, a vast array of hypoxia induced metabolic and molecular responses are already characterised by us. Now, we want to investigate the sensing and signalling cascade that is involved in regulating the various responses. We will use RT-PCR transcription factor profiling to identify oxygen related transcription factors that are involved in regulating gene expression and adaptation to low oxygen. The selected transcription factors will be characterised in-vivo and in-vitro to reveal their physiological function and their genetic interaction to trigger transcription of oxygen sensitive promoters. Target sequences will be explored in chromosomal sections that have been identified by QTL analysis of the low-oxygen response. A luciferase reporter screen is used to find oxygen signalling mutants defective in genes that link the changes in gene expression to low oxygen.