SBIR Phase I: Rapid Antibiotic Susceptibility Testing

这项小型企业创新研究（SBIR）第一阶段提出开发一种具有无与伦比的速度和简单性的抗生素敏感性测试新方法。 该技术方法基于阻抗感测的新实现，以前所未有的灵敏度和稳定性监测细胞生长。 这使得能够在不到一小时的时间内直接从细菌悬浮液中获得结果，并且使用简单、廉价且易于使用的设备，非常适合大规模生产。 与目前使用的方法相比，这种方法避免了培养的需要，减少了从样品收集到完成诊断的时间。 在第一阶段的研究中，我们会验证建议的诊断工具在大肠杆菌和枯草芽孢杆菌的抗药性菌株上的可行性，并将建议产品的商业应用作为一种诊所内的医疗诊断工具。 生物样品的低成本和易于使用的快速和特异性监测器在整个微生物测试行业中具有广泛的应用。 具体的大市场包括尿路感染的快速技术，分枝杆菌的测试和生物威胁剂的测试。

This Small Business Innovation Research (SBIR)Phase I proposes to develop a new method for antibiotic susceptibility testing having unparalleled speed and simplicity. The technical approach is based on a novel implementation of impedance sensing to monitor cellular growth with unprecedented sensitivity and stability. This enables results to be obtained directly from bacterial suspensions in less than one hour with a simple, inexpensive, and easy to use device ideally suited for mass production. This method avoids the need for growing cultures reducing the time from sample collection to complete diagnosis by weeks compared to currently used methods. In this Phase I project, the feasibility of the proposed diagnostic tool with antimicrobial drug resistant strains of Escherichia coli and Bacillus subtilis will be demonstrated.The commercial application of the proposed product will be as an in-office medical diagnostic. A low-cost and easy-to-use rapid and specific monitor of biological samples has broad application throughout the microbiological testing industry. Specific large markets include rapid techniques for urinary tract infections, testing of mycobacteria, and testing of biological threat agents.