Apple Functional Genomics

该项目的总体目标是建立苹果基因组的结构工具。苹果是美国最重要的落叶果树作物。该项目将致力于开发结构基因组研究工具，重点研究苹果的开花、结果和病原菌响应。该项目的第一阶段是构建一个包含12万个序列的表达序列标签(EST)数据库，该数据库来自开花和结果组织，以及响应病原菌侵染的组织，特别是对细菌病原菌淀粉欧文氏菌(引起火疫病的病原菌)和真菌病原菌Venturia inaequalis(苹果赤霉病的病原菌)。然后，这些克隆将被用来产生一组30,000个Unigene克隆，这些克隆将进行3‘测序。苹果基因组数据集将被生成并整合到国家蔷薇科基因组数据库中。该项目的教育和推广部分将涉及开发基于网络的模块，用于对高中教师和学生进行结构基因组学概念的教育。

AbstractThe overall goal of this NSF project is to establish structural tools for apple genomics.The apple, Malus x domestica Borkh., is the most important deciduous tree fruit cropgrown in the United States. This project will focus on developing structural genomicresearch tools with emphasis on flowering, fruiting, and pathogen response in apple.The first phase of this project is to construct an expressed sequence tag (EST) databaseof 120,000 sequences from flowering and fruiting tissues, and from tissues responding topathogen infection, specifically to the bacterial pathogen Erwinia amylovora (the causalorganism of fire blight disease) and to the fungal pathogen Venturia inaequalis (thecausal organism of apple scab disease). These will then be used to generate a set of30,000 unigene clones that will be subjected to 3' sequencing. An apple genome dataset will begenerated and integrated into a national Rosaceae Genome Database. Aneducational and outreach component of this project will involve developing web-basedmodules for use in educating high school teachers and students on the conceptsstructural genomics.