Acquisition of a Nanoelectrospray Source and Supporting Equipment

在化学研究仪器和设施（CRIF）计划的支持下，伦斯勒理工学院化学系将购买纳米电喷雾（nano-ESI）源和配套设备。这台设备将被放置在RPI的质谱设施中，将加强许多领域的研究，包括1）在色谱分离过程中确认蛋白质置换分子的功效; B）从头设计蛋白质和设计方法以增强肽的质谱信号; c）分析寡糖的复杂混合物; d）重组蛋白质生产;和e）阐明蛋白质在固定的生物材料上的沉积。质谱（MS）是一种用于探测紧密结构细节并获得大量有机、生物有机和有机金属分子的分子组成的技术。 Nano-ESI是一种经过验证的方法，用于电离感兴趣的分子而不发生碎片化。 因此，MS和nano-ESI的组合是增长最快和最广泛使用的分析仪器技术之一，用于较大的生物分子，否则这些生物分子会在电离时断裂。 这些研究将在许多领域产生重大影响，特别是生物化学。

With support from the Chemistry Research Instrumentation and Facilities (CRIF) Program, the Department of Chemistry at Rensselaer Polytechnic Institute will purchase a nanoelectrospray (nano-ESI) source and supporting equipment. This equipment, which will be placed in the Mass Spectrometry Facility at RPI, will enhance research in a number of areas including 1) confirmation of the efficacy of protein displacement molecules during chromatographic separations; b) de novo protein design and design of methodologies to enhance mass spectrometric signals from peptides; c) analysis of complex mixtures of oligosaccharides; d) recombination protein production; and e) elucidation of protein deposition on explanted biomaterials. Mass spectrometry (MS) is a technique used to probe intimate structural details and to obtain the molecular compositions of a vast array of organic, bioorganic, and organometallic molecules. Nano-ESI is a proven method for ionizing the molecule of interest without fragmentation. Thus the combination of MS and nano-ESI is one of the fastest growing and most widely used analytical instrumentation techniques for larger biomolecules that would otherwise fragment upon ionization. These studies will have a significant impact in a number of areas, especially biochemistry.