The role of sulfotransferase proteins in the glucosinolate biosynthesis of Arabidopsis thaliana

硫转移酶（SOT, EC 2.8.2）的所有成员。-)蛋白家族使用3 ' -磷酸腺苷5 ' -磷酸硫酸作为硫酰供体，并将硫酸基团转移到几类底物的适当羟基上。拟南芥全基因组测序共包含21个可能编码SOT蛋白的基因。植物SOT蛋白的大多数底物及其生理作用尚不清楚。其中三个催化硫代葡萄糖苷（GL）生物合成的最后一步。我们想继续在体外鉴定不同拟南芥生态型的重组desulfo (ds)-GL SOT蛋白。这些研究的一个重点是了解更多的反应机制。此外，SOT蛋白的三维结构可以帮助理解为什么同种异构体具有不同的底物特异性。此外，ds-GL AtSOT蛋白的作用必须通过对不同植物突变体的体内分析来证实。因此，GL模式将在可用T-DNA插入和ds-GL AtSOT RNAi突变体中进行分析。该结果有助于更好地了解拟南芥的GL代谢。从模型系统拟南芥的见解转移将转移到作物植物甘蓝型油菜。

All members of the sulfotransferase (SOT, EC 2.8.2.-) protein family use 3’-phosphoadenosine 5’-phosphosulfate as sulfuryl donor and transfer the sulfate group to an appropriate hydroxyl group of several classes of substrates. The fully sequenced genome of Arabidopsis thaliana contains in total 21 genes that are likely to encode SOT proteins. Most of the substrates, and therefore the physiological roles, of plant SOT proteins are not known. Three of them catalyze the last step of glucosinolate (GL) biosynthesis. We would like to continue the in vitro characterization of recombinant desulfo (ds)-GL SOT proteins from different Arabidopsis ecotypes. One focus in these studies is to learn more about the reaction mechanism. Additionally, a three-dimensional structure of a SOT protein can help to understand why the isoforms have different substrate specificities. Furthermore the role of ds-GL AtSOT proteins has to be confirmed in vivo by the analysis of different plant mutants. Therefore the GL pattern will be analysed in available T-DNA insertion and in ds-GL AtSOT RNAi mutants. The results could lead to a better understanding of GL metabolism in Arabidopsis. A transfer of insights from the model system Arabidopsis will be transferred on the crop plant Brassica napus.