Acquisition of a Quantitative Gene Detection and Expression System

在Brian Rogerson博士的指导下，新泽西州理查德斯托克顿学院获得了一笔赠款，用于获得定量基因检测和表达系统。此次收购项目将增强我们核心分子分析设施的能力，该设施将用于支持生物和化学项目的本科生研究、教师研究和教学，以及与学院以外的研究人员的合作研究。将受益于这项技术的研究项目包括：(1)检查结核分枝杆菌对表现出不同程度耐药性的受感染小鼠宿主肺部生命的适应性反应（Rogerson博士）；(2)研究污染对冬季比目鱼基因表达的影响（Straub博士），该项目正在进行中，以支持当地渔业研究，并将来自受污染严重的拉坦/哈德逊河口与受污染较少的地区的个体鱼进行比较。(3)蛋白磷酸酶2A（一种高度保守的细胞酶）及其在酵母Saccharomyces cerevisiae中调控基因表达的作用的研究（Dr. York）。该仪器还将使基因表达实验更容易整合到高级实验课程中，如生物技术（Straub博士）、分子遗传学（York博士）和发育生物学（Herlands博士）。在我们的本科生研究实验室增加定量序列检测系统是我们继续努力为学生提供相关培训机会的一部分，这些培训将为他们在技术部门的工作或为他们的研究生和专业培训做好准备。这将帮助我们实现新泽西州高等教育委员会的目标之一。该项目有望有助于我们理解：(1)细菌对哺乳动物免疫反应的适应性；(2)用于调查鱼类栖息地的适宜性、产量或污染程度的生物标志物；(3)酵母等模式生物如何揭示基因调控等基本细胞过程。这个收购项目也将有助于保持我们的核心设施作为与其他机构的研究人员合作的焦点。

A grant has been awarded to The Richard Stockton College of New Jersey under the direction of Dr. Brian Rogerson for the acquisition of a quantitative gene detection and expression system. This acquisition project will enhance the capabilities of our Core Molecular Analysis Facility, which is being used to support undergraduate student research, faculty research and teaching in the biology and chemistry programs, as well as collaborative research efforts with investigators outside the college. Research projects that will benefit from this technology include: (1) The examination of the Mycobacterium tuberculosis adaptive response to life in the lungs of infected mouse hosts exhibiting different levels of resistance (Dr. Rogerson), (2) the study of pollution effects on gene expression in winter flounder (Dr. Straub), a project that is being carried out in support of local fisheries research and that will compare individual fish from the highly pollution-impacted Raritan/Hudson Estuary with less impacted sites, and (3) the study of protein phosphatase 2A, a highly conserved cellular enzyme, and its role in regulating gene expression in the yeast Saccharomyces cerevisiae (Dr. York). This instrumentation will also allow gene expression experiments to be more easily integrated into upper level laboratory courses such as Biotechnology (Dr. Straub), Molecular Genetics (Dr. York) and Developmental Biology (Dr. Herlands). Addition of a quantitative sequence detection system to our undergraduate research laboratories is part of our continuing effort to provide relevant training opportunities for students that will prepare them for jobs in the technology sector or prepare them for graduate and professional training. This will help us fulfill one of the goals of the New Jersey Commission on Higher Education. This project is expected to contribute to our understanding of: (1) bacterial adaptation to the immune response in mammals, (2) biomarkers for surveying fish habitats for suitability, production or degree of contamination, and (3) how model organisms such as yeast can shed light on basic cellular processes such as gene regulation. This acquisition project will also help maintain our core facility as a focal point for collaboration with researchers at other institutions.