Employing Differential Scanning Calorimetry and Laser Light Scattering For a Thermodynamic-Rich Undergraduate Chemistry Curriculum

化学(12)购置了差示扫描量热计(DSC)和激光光散射分光光度计(LLS)，从而将物理化学、生物化学、生物物理化学和独立研究课程与生物相关、热力学丰富的实验室经验相结合。改编自教育文献的LLS和DSC实验正在实施，其中包括合成聚合物、蛋白质、核酸或类脂膜的热力学表征。例如，在物理化学课程中，学生使用最小二乘法测定聚苯乙烯-甲基丙烯酸甲酯聚合物的相对分子质量，并表征其与有机溶剂的相互作用。用差示扫描量热法(DSC)表征了共聚物的玻璃化转变以及磷脂双层相变。生物化学学生使用DSC和LLS来表征实验室中分离和纯化的T7聚合酶，并将这些研究与蛋白质的功能属性联系起来。生物物理化学专业的学生使用LLS和DSC两种独立的研究形式来研究核酸双链体形成的热力学及其对离子强度和溶剂质量的依赖。LLS和DSC仪器也用于与同事和本科生的合作研究。这些仪器的使用为学生提供了舒适和熟悉热力学的机会，同时应对了Bio2010的挑战。

Chemistry (12)Acquisition of a differential scanning calorimeter (DSC) and a laser light scattering (LLS) spectrophotometer is resulting in the infusion of the physical chemistry, biochemistry, biophysical chemistry, and independent research courses with biologically relevant, thermodynamic-rich laboratory experiences. LLS and DSC experiments adapted from the educational literature that incorporate the thermodynamic characterization of synthetic polymers, proteins, nucleic acids, or lipid membranes are being implemented. For example, in the physical chemistry course students use LLS to determine the molecular weight of a polystyrene-methyl methacrylate polymer and characterize its interactions with organic solvent. DSC is used to characterize the glass-transition of the copolymer as well as a phospholipids bilayer phase transition. Biochemistry students use DSC and LLS to characterize T7 polymerase isolated and purified in the lab and relate these investigations to the functional attributes of the protein. Biophysical chemistry students use both LLS and DSC in an independent, investigative format to study the thermodynamics of nucleic acid duplex formation and its dependence on ionic strength and solvent quality. The LLS and DSC instruments are also used in collaborative research with colleagues and undergraduate students. The use of these instruments provides student comfort and familiarity with thermodynamics while addressing the Bio2010 challenge.