Acqusition of Genonics Instrumentation for Evolutionary, Ecological, and Environmental Biology.

该合同将支持基因组学仪器的收购，包括高通量实时PCR和微阵列打印和扫描。这些仪器将加强以下项目：(1)适应性基因复合物的进化；(2)昆虫摄食和其他环境应激源对植物整体基因表达的影响；(3)人工湿地微生物和根系群落结构分析；(4)蓝藻毒素的毒物基因组学和有害藻华的基因组学和蛋白质组学特征。该提案的目标是建立一个基因组学设施，用于研究非模式生物，这是莱特州立大学正在进行的研究项目的主题。使用非模式生物是进化、生态和环境生物学研究的一个重要方面。基因组学已经彻底改变了生物科学研究的方式。基因组学技术在环境和生态研究中的应用有可能彻底改变这些领域，因为它已经彻底改变了分子和细胞生物学。例如，该奖项使一个项目成为可能，该项目分析了为处理过氯乙烯及其致癌降解产物氯乙烯而建造的湿地中的细菌群落。该湿地的细菌群落结构和演替特征将有助于设计用于处理其他有机化合物的湿地。另一个项目将是对藻华的基因组分析，这将允许快速区分有毒和无毒的藻华。第三个项目将需要开发利用单核苷酸多态性进行遗传作图的高通量方法。获得的仪器的技术创新将集成到研究生和本科教育计划中，包括针对代表性不足群体的计划。最近在这一领域的努力包括资助了环境生物学本科生指导（UMEB）、弱势群体环境压力研究（Environmental Stress for the Underrepresented）和为残疾理科生创造实验室通道（CLASS）等项目。这些学生中的许多人继续在生物科学系的研究生课程中接受教育。其中包括2002年成立的环境科学博士项目。环境科学是赖特州立大学的一个卓越领域。

This award will support the acquisition of genomics instrumentation including high-throughput real-time PCR and microarray printing and scanning. These instruments will enhance projects that include the following: (1) the evolution of adaptive gene complexes (2) the effects of insect feeding and other environmental stressors on global gene expression in plants, (3) analyses of microbial and root community structures in constructed wetlands, and (4) toxicogenomics of cyanobacterial toxins and genomic and proteomic characterizations of harmful algal blooms. The goal of this proposal is the establishment of a genomics facility that will be used for research on nonmodel organisms that are the subject of ongoing research programs at Wright State University. The use of nonmodel organisms is a crucial aspect in studies of evolutionary, ecological, and environmental biology.Genomics has revolutionized the way research is conducted in Biological Sciences. Application of genomics technology to environmental and ecological studies has the potential to revolutionize these fields as it has revolutionized molecular and cellular biology. For example, one project made possible by this award is the analysis of bacterial communities in a wetland constructed for the treatment of perchloroethylene and its carcinogenic degradation product, vinyl chloride. Characterization of bacterial community structure and succession in this wetland will help in the design of wetlands constructed for the treatment of other organic compounds. Another project will be genomic analyses of algal blooms, which will allow for rapid discrimination between toxic and nontoxic blooms. A third project will entail the development of high throughput methods for genetic mapping using single nucleotide polymorphisms. The technical innovations from the acquired instrumentation will be integrated into graduate and undergraduate educational programs, including programs that target underrepresented groups. Recent efforts in this area include funded projects for Undergraduate Mentoring in Environmental Biology (UMEB), Research in Environmental Stress for the Underrepresented, and Creating Laboratory Access for Science Students with Disabilities (CLASS). Many of these students continue their education in the graduate programs of the Department of Biological Sciences. Among these is an Environmental Sciences Ph.D. program that was established in 2002. Environmental Sciences is an area of excellence at Wright State.