MRI/RUI Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Chemistry Research at California State University, Northridge

在主要研究仪器(MRI)计划的这一奖项下，位于北岭的加州州立大学化学系将获得一台液体色谱仪离子陷阱串联质谱仪(LC/MS)。五个研究领域将立即从所要求的LC-MS仪器中受益：(1)转移加氢催化剂的直接设计。LC-MS将用于确认关键的反应中间体(特别是氢化Ru和催化剂-底物加合物)以及新催化剂及其产物的表征。(2)了解有机声化学中的基本分子过程。LC-MS有助于从热自由基和羟基自由基途径确定中间体结构，并区分潜在的机理。(3)芳香酶诱导的多功能1，5-烷基二炔类抗肿瘤药物的代谢。LC-MS对于确认芳香酶与药物的相互作用、鉴定代谢物和测试前药设计以靶向传递有希望的药物治疗乳腺癌将是重要的。(4)信使核糖核酸和蛋白质的同时分析。LC-MS将用于从大量裂解液样品中分离和鉴定目标蛋白(从乳腺癌细胞中的雌激素受体开始)。(5)载脂蛋白E糖基化程度和部位的评价。LC-MS将用于确认高葡萄糖浓度下THP-1巨噬细胞分泌的载脂蛋白E的蛋白分解片段中的果糖赖氨酸残留量。液质联用(LC/MS)是一种用于复杂混合物分离和分析的非常有效的技术。这一仪器将大大加强加州州立大学北岭分校的科学研究，这是一所以本科为主的机构。这些研究将在生物化学和药物化学领域产生坚实的影响。

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at California State University in Northridge will acquire a liquid chromatograph ion trap tandem mass spectrometer (LC/MS). Five areas of research will immediately benefit from the requested LC-MS instrumentation: (1) Direct design of transfer hydrogenation catalysts. LC-MS will be used in confirmation of key reaction intermediates (especially ruthenium hydrides and catalyst-substrate adducts) as well as characterization of new catalysts and the products they produce. (2) Understanding fundamental molecular processing in organic sonochemistry. LC-MS will facilitate the determination of intermediate structures from thermal and hydroxyl radical pathways and distinction of the underlying mechanisms. (3) Aromatase-induced metabolism of poly-functional 1,5-alkadiyne anti-tumor agents. LC-MS will be important to confirm aromatase-drug interactions, identify metabolites and test pro-drug designs for targeted delivery of promising agents in breast cancer. (4) Simultaneous analysis of mRNA and protein. LC-MS will be used in the separation and identification of target proteins (beginning with estrogen receptor in breast cancer cells) from bulk lysate samples. (5) Evaluation of the extent and sites of apolipoprotein E glycation. LC-MS will be used to confirm fructoselysine residues within proteolytic fragments of apolipoprotein E secreted from THP-1 macrophages at elevated glucose concentrations. Liquid chromatography with mass spectrometric detection (LC/MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at California State University in Northridge, a primarily undergraduate institution. These studies will have a solid impact in the area of biochemistry and medicinal chemistry.