CAREER: CHEMICAL METHODS FOR SELECTIVE PROTEOME ANALYSIS

Gavin E.密歇根州立大学的Reid得到化学系分析和表面化学项目的支持，开发了一系列新的衍生化策略，这些策略将允许将“固定电荷”掺入肽内的目标氨基酸残基，从而能够从蛋白质酶的复杂混合物中选择性地鉴定和定量特定的肽组。 该项目将有助于气相电荷导向肽片段化的基础知识，并将增加新的试剂和分析工具，通过质谱法进行蛋白质鉴定。 该项目的工业推广部分预计将积极影响参与该项目的研究生和本科生。质谱作为一种主要的生物分析工具的出现是仪器创新和可应用于复杂生物分子的新电离化学的产物。 这项工作将在分析化学、生物化学和生物学领域产生重大影响。

Professor Gavin E. Reid of Michigan State University is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to develop a range of new derivatization strategies that will allow the incorporation of a "fixed charge" onto targeted amino acid residues within peptides, to enable selective identification and quantitation of specific groups of peptides from complex mixtures of protein digests. The project will contribute to the fundamental knowledge in gas phase charge-directed peptide fragmentation, and will add new reagents and analytical tools for protein identification by mass spectrometry. The industrial outreach component of this project is expected to positively impact graduate and undergraduate students involved in this project.The emergence of mass spectrometry as a major bioanalytical tool is the product of both innovations in instrumentation and new ionization chemistries that can be applied to complex biomolecules. This work will have significant impact in areas of analytical chemistry, biological chemistry, and biology.