IODP Microbiology Through Massively Parallel DNA Sequencing

该计划旨在研究新的大规模并行DNA测序技术的应用，以实现环境微生物学目标，特别关注IODP下的高优先级深层生物圈研究。 使用ODP Leg 201（秘鲁深层生物圈钻探）1229号站点的5个样本，研究人员将进行概念验证研究，以DNA测序，重点关注传统措施认为DNA贫乏的材料。 他们将首先为五个样本中的每一个产生大约300，000个100个碱基对（~30 Mb）的序列，然后绘制这些宏基因组。 在研究的第二阶段，他们将对1229号研究中心的重要水平线或另一个孔的样本进行重点研究，并在3或4轮测序中产生约90 Mb的额外基因组DNA数据。 这项研究的主要广泛影响是它有可能彻底改变微生物生态学，特别是对于难以研究的深海生物圈样本。 此外，该研究将支持一名研究生，并加强地质微生物学家和微生物生态学家之间的跨学科合作。

This program seeks to investigate the application of newly available, massively parallel DNA sequencing technology for the pursuit of environmental microbiology objectives, with particular attention to high-priority deep biosphere studies under IODP. Using 5 samples from Site 1229 of ODP Leg 201 (Peruvian deep biosphere drilling), the researchers will conduct a proof-of-concept study to sequence DNA focusing on material that is considered DNA-poor by conventional measures. They will first produce an estimated 300,000 sequences of 100 base pairs (~30 Mb) for each of the five samples and then map these metagenomes. During a second phase of the study, they will conduct a focused study either on an important horizon at Site 1229 or on samples from another hole and produce ~90 Mb of additional genomic DNA data in 3 or 4 rounds of sequencing. The primary broader impact of this research is its potential to revolutionize microbial ecology, particularly for difficult-to-study samples of the deep marine biosphere. In addition, the research will support a graduate student and strengthen the interdisciplinary collaboration between a geomicrobiologist and a microbial ecologist.