Self-Referenced SPR Sensing using Multiple Surface-Plasmon Modes

杰弗里·T·黑斯廷斯肯塔基州大学的杰弗里·T·黑斯廷斯摘要智能优点：表面等离子体共振已经成为一种广泛使用的、无标记的技术，用于检测和研究生物和化学相互作用。然而，一个根本的挑战仍然没有解决：人们如何区分非特定的影响(温度波动、溶液折射率变化、干扰物的非特定结合等)。以及检测到目标分析物？这一问题目前限制了表面等离子体共振在复杂生物样品以及需要现场可部署传感器的医疗、环境、食品安全和国防应用中的有效性。这项研究工作通过开发一种基于同时耦合到多个表面等离子激元模式的自参考传感器平台来应对这一挑战。首次设计了微结构支持的表面等离子体波的电磁场分布和色散关系，以区分多种生物和化学过程。将这种自我参照的方法与传感器表面抗体的定向固定相结合，将提供一种系统，选择性地检测复杂溶液中致病菌的存在，如大肠杆菌和炭疽芽孢杆菌。更广泛的影响：新的传感器平台将更好地服务于药物发现、医疗诊断、食品质量保证和生化防御方面的社会需求。从教育的角度来看，该项目在一个固有的跨学科领域提供研究生培训，同时加强肯塔基大学纳米科学与工程中心的多学科伙伴关系。该项目也是针对肯塔基州阿巴拉契亚地区高中生的多样性推广工作的示范平台，以及与肯塔基大学本科纳米工程证书计划(NECP)相关的新的微光和纳米光子学本科课程。

Jeffrey T. HastingsUniversity of Kentucky0601351AbstractIntellectual Merit: Surface-plasmon resonance has become a widely used, label free technique to detect and study biological and chemical interactions. Nevertheless, a fundamental challenge remains unresolved: How does one differentiate between non-specific effects (temperature fluctuations, solution refractive index changes, non-specific binding of interferents, etc.) and detection of a target analyte? This problem currently limits the effectiveness of surface-plasmon resonance in complex biological samples and for medical, environmental, food safety, and defense applications that require field deployable sensors. This research effort addresses this challenge by developing a self-referencing sensor platform based on simultaneous coupling to multiple surface-plasmon modes. For the first time, the electromagnetic field distributions and dispersion relations of surface-plasmon waves supported by microstructures will be engineered to distinguish multiple biological and chemical processes. Combining this self-referencing approach with oriented immobilization of antibodies on the sensor surface will provide a system that selectively detects the presence of pathogenic bacteria such as Escherichia coli and Bacillus anthracis in complex solutions. Broader Impacts: The new sensor platform will better serve society's needs in drug discovery, medical diagnosis, food quality assurance, and bio-chemical defense. From an educational perspective, the project provides graduate training in an inherently interdisciplinary field while strengthening multi-disciplinary partnerships in the University of Kentucky's Center for Nanoscale Science and Engineering. The project also serves as a demonstration platform for a diversity outreach effort targeting high-school students from Kentucky's Appalachian regions and for a new Micro- and Nano-Photonics undergraduate course offered in connection with the University of Kentucky's undergraduate Nanoscale Engineering Certificate Program (NECP).