Thylakoid membrane biogenesis in Chlamydomonas reinhardtii

叶绿体包含一个复杂的膜网络，称为类囊体，其中三个完整的膜复合物进行光合作用电子传递和质子易位。类囊体膜的生物形成需要多种协调一致的生化途径以及细胞器和核基因组多肽的表达。这些过程的精确位置是一个长期存在的问题。我建议用细胞生物学、遗传学和蛋白质组学的方法来描述一种新的类囊体生物发生室和莱茵衣藻和植物模式生物叶绿体中类囊体生物发生的动力学。这些结果具有很高的潜力，为研究类囊体的生物发生、膜运输、光系统组装、蛋白质合成和靶向提供细胞学背景。我还希望揭示膜生物发生的一般原理。

Chloroplasts contain an elaborate network of membranes, called thylakoids, where three integral membrane complexes carry out photosynthetic electron transport and proton translocation. The biogenesis of thylakoid membranes requires multiple concerted biochemical pathways and the expression of the polypeptides from the organelle and nuclear genomes. A long-standing problem concerns the precise location(s) of these processes.I propose to characterize a novel thylakoid biogenesis compartment and the dynamics of thylakoid biogenesis in the chloroplasts of the green alga Chlamydomonas reinhardtii and plant model organisms using cell biological, genetic, and proteomic approaches. The results have a high potential to provide a cytological context in which to study in thylakoid biogenesis as well as membrane trafficking, photosystem assembly, and protein synthesis and targeting. I also expect to reveal general principles underlying membrane biogenesis.