Biochemical characterization of fruiting body formation in the myxobacterium Myxococcus xanthus

拟议的项目涉及在饥饿条件下形成子实体的粘杆菌黄粘球菌的生理学。在这些子实体中，营养细胞发育成抗逆性黏液孢子。为了了解潜在的过程，并确定这一发展的特征和/或必要的生物标志物，应该研究几类化合物（脂肪酸、碳水化合物、氨基酸）组成的定性和定量变化。因此，这项工作是详细的代谢通量分析的基础，这对于优化选择的代谢途径至关重要，特别是关于生产具有重要药用意义的化合物（抗生素，抗癌化合物），其形成是典型的黏菌。

The proposed project deals with the physiology of the myxobacterium Myxococcus xanthus that forms fruiting bodies under starvation conditions. In these fruiting bodies vegetative cells develop into stress-resistant myxospores. In order to understand the underlying processes and to identify biomarkers that are characteristic and/or essential for this development, qualitative and quantitative changes in the composition of several classes of compounds (fatty acids, carbohydrates, amino acids) should be investigated. Thus this work is the basis for a detailed metabolic flux analysis which is essential to optimize selected metabolic pathways especially with respect to the production of pharmaceutically important compounds (antibiotics, anti-cancer compounds), whose formation is typical for myxobacteria.