Minority Postdoctoral Research Fellowship for FY2006

该项目是根据2006年少数民族博士后研究奖学金和支持活动计划授予的。蛋白质对几乎每一个生物过程都是必不可少的，生产具有规定性质的新蛋白质具有解决无数医学和环境问题的潜力。然而，一般蛋白质设计的实用方法超出了平均长度蛋白质的任何实际应用。因此，本次调查的目标是有效地巩固功能的可扩展性（即，折叠、组装、结合、变构机制）在单一系统中-蛋白质乳糖阻遏物。使用这个统一的平台，具有规定的变构和结合特性的原型阻遏物将被重新设计。这些研究的结果将最终导致更有效地解释实验结果，并开发新的方法，以更彻底地分析和设计其他蛋白质家族。培训目标，职业目标和影响：作为加州理工学院的博士后学者与博士。马约和乔纳森J.西尔伯格，威尔逊博士将开发联合方法（即，实验的和理论的）来定义单个系统中的折叠、结合和功能（变构）关系。利用这些信息，一个新的，重新设计的一套转录抑制剂与规定的属性将被开发。最终，威尔逊博士的职业目标是在高等院校（顶尖大学）获得一个学术职位，在那里他将致力于解决有关蛋白质序列，结构和直接生物医学和生物技术相关的功能关系的基本科学问题。此外，作为一名独立的科学家，他愿意参加对未来科学调查人员的教育和培训。

This project is awarded under the Minority Postdoctoral Research Fellowships and Supporting Activities Program for 2006. Proteins are essential to virtually every biological process and the production of new proteins with prescribed properties has the potential of solving countless medical and environmental problems. However, a practical approach to general protein design is beyond any realistic application for average length proteins. Thus, the goal of this investigation is to effectively consolidate the requisites for function (i.e., folding, assembly, binding, allosteric mechanisms) in a single system-the protein lactose repressor. Using this consolidated platform the prototypic repressor with prescribed allosteric and binding properties will be redesigned. The results from these studies will ultimately result in more effective interpretations of experimental results and the development of new approaches for more thorough analysis and design in other protein families.Training Objectives, Career Goals, And Impact:As a postdoctoral scholar at Cal Tech with Drs. Stephen L. Mayo and Jonathan J. Silberg, Dr. Wilson will develop combined approaches (i.e., experimental and theoretical) to define the folding, binding, and function (allosteric) relationships in a single system. Using this information, a novel, redesigned set of transcriptional repressors with prescribed properties will be developed. Ultimately, Dr. Wilson's career objective is to obtain an academic position at an institute of higher learning (a top university) where he will work to address fundamental scientific questions about protein sequence, structure, and function relationships of direct biomedical and biotechnological relevance. Furthermore, as an independent scientist, he would like to participate in the education and training of future scientific investigators.