RUI: Model Compounds for P460 from Hydroxylamine Oxidoreductase

圣弗朗西斯大学化学、数学和物理科学系的Edward P. Zovinka教授得到了无机、生物无机和有机金属化学项目的支持，研究欧洲亚硝化单胞菌（Ne）中羟基胺氧化还原酶（HAO）的模型，这是一种自养土壤细菌，通过氧化含氮物质来产生能量。HAO含有一种独特的酪氨酸-卟啉连接，据推测，这种连接在酶的活性中起重要作用。为了更好地了解活性位点的化学性质，本科研究人员合成并研究了含有中位（羟基苯基）卟啉配体作为卟啉-酪氨酸部分类似物的模型化合物。卟啉配体与具有重要生物学意义的铁配合，并研究了卟啉配体与羟胺的反应性。该小组利用绿色化学原理合成配体。这包括使用微波方法来提高反应收率和减少反应时间。通过文献讨论，向所有参与研究的本科生强调科学伦理。

Professor Edward P. Zovinka, Department of Chemistry, Mathematics, and Physical Science, Saint Francis University is supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program to study models for hydroxylamine oxidoreductase (HAO) in Nitrosomonas europea (Ne), an autotrophic soil bacteria that oxidizes nitrogenous materials in order to make energy. HAO contains a unique tyrosine-porphyrin connection that has been speculated to play an important role in the enzyme''s activity. To better understand the chemistry of the active site, undergraduate researchers synthesize and study model compounds containing meso-(hydroxyphenyl)porphyrin ligands as analogs for the porphyrin-tyrosine moiety. The porphyrin ligands are coordinated to biologically significant iron and the reactivities of the compounds with hydroxylamine are studied. The group utilizes Green Chemistry principles in the synthesis of the ligands. This includes the use of microwave methods to improve reaction yields and decrease reaction times. Scientific ethics are stressed to all participating undergraduate researchers through literature discussions.