Collaborative Research: Advanced Laser Fluorometer (ALF) for in vivo Characterization of Phytoplankton Pigments, Physiology and Community Structure

提供的资金使项目能够进入下一阶段的长期努力，开发先进激光荧光（ALF）技术，用于从不同平台测量一系列海洋环境中的关键生物地球化学变量和浮游植物群落结构。该方法建立在激光和传感器技术的最新进展的基础上，这些技术最近产生了一些新的发展，包括激光机载传感器和用于生物环境河口表征的紧凑型ALF-1荧光计。ALF-2的开发将提供高分辨率的船上航行流量测量和一系列空间和时间尺度的样本分析。为了指导技术发展并为跨学科海洋观测提供新的信息，ALF技术将被整合到加州当前生态系统长期生态研究（NSF）、加州海洋渔业合作调查（CalCOFI， NOAA）、微生物观测计划（NSF）的过程研究和运行监测中，并为有害藻华计划（NOAA）进行评估。更广泛的影响如果AFL-2被验证并提供给研究界，它将成为生物光学和浮游植物生态学的一个有价值的工具。它也可以成为实验室里重要的教学工具。该提案包括培养两名博士后研究人员和支持本科生研究。

Funding was provided to enable the PIs to move to the next phase in of a long-term effort to develop Advanced Laser Fluorescence (ALF) technology for measurements of key bio-geochemical variables and phytoplankton community structure in a range of marine environments from different platforms. The approach builds upon recent advances in laser and sensor technology that have recently resulted in several new developments, including the laser airborne sensors and the compact ALF-1 fluorometer for bio-environmental estuarine characterization. The ALF-2 development will provide high-resolution shipboard underway flow-through measurements and sample analyses over a range of spatial and temporal scales. To guide the technology evolution and provide new information to interdisciplinary ocean observations, ALF technology will be integrated into the process studies and the operational monitoring of the California Current Ecosystem Long Term Ecological Research (NSF), California Cooperative Oceanic Fisheries Investigations (CalCOFI, NOAA), Microbial Observatory program (NSF) and evaluated for the Harmful Algal Bloom program (NOAA). Broader ImpactsThe AFL-2 could become a valuable tool in bio-optics and phytoplankton ecology if validated and made available to the research community. It could also become an important teaching tool in the lab. The proposal includes training of two postdoctoral researchers and support for undergraduate research.