SBIR Phase I: Cryopreserved Cells as Reagents

这项小企业创新研究（SBIR）第一阶段研究项目旨在开发一种冷冻细胞的系统，以避免解冻后培养和细胞扩增的需要。如果成功，这项技术将对从事任何依赖组织培养的生物研究方面的科学家具有重要价值，包括高通量分析。目前使用冷冻细胞的方法要求将细胞从冷冻室取出后进行扩增。这不仅耗时，而且细胞活力可能会变化，从而影响培养实验所需细胞数量所需的时间。所提出的方法，基于使用顺磁负载的生物相容性珠粒，将允许细胞在珠粒上生长，并使冷冻到实验室工作台的能力。因此，这项技术将对高通量分析和其他需要细胞培养进行实验的领域产生深远的影响。

This Small Business Innovation Research (SBIR) Phase I research project aims to develop a system for the freezing of cells that obviates the need for post-thaw culture and expansion of the cells. If successful, the technology would be of significant value to scientists involved in any aspect of biological inquiry that relies on tissue culture, including high-throughput assays. Current methods for the use of frozen cells require that the cells be expanded after removal from the freezer. This is not only time-consuming, but cell viability can vary and thus influence the amount of time that it takes to grow the needed number of cells for the experiment. The proposed methodology, based on the use of paramagnetic loaded biocompatible beads, would allow cells to grow on the beads and enables freezer-to-lab bench capability. As such the technology would have a profound impact on high-throughput assays and other areas where cell culture is needed for experimentation.