IDBR: Development of a Multivariate Hyper-Spectral Instrument for High-Resolution Chemical Imaging of Cell Structure and Dynamics

普渡大学的本·阿莫茨博士获得了一笔赠款，用于开发一种高速化学成像仪器，该仪器将跟踪活细胞中的分子。目前的化学成像仪器受到高通量（速度）和高信息含量（分辨率）之间的权衡的限制。这种新的多变量高光谱成像（MHI）仪器将克服这些限制，使用一种新的数据压缩策略，以产生一个高速的“化学视觉”系统。换句话说，MHI仪器将拍摄照片和电影，揭示特定分子的位置以及它们如何移动。除了细胞成像之外，预期MHI可用于多种其它生物、医学和环境应用，如下文进一步描述。MHI仪器可以被看作是第一个新品种的化学成像光谱仪，快速收集全光谱图像使用多元数据压缩。这种数据压缩策略与常用的音乐和视频数据压缩方法有关。然而，一个关键的区别是，MHI使用数据压缩来加速高光谱图像的收集。这是通过使用高分辨率空间光调制器（SLM）来快速检测全光谱响应函数来实现的。所得光谱响应强度可用于直接对细胞和细胞组分成像或重建完整的高分辨率光谱图像。这种硬件数据压缩策略有望将光谱成像速度提高1000倍，从而促进单个活细胞的高分辨率化学成像和动力学研究，以及高通量/高含量细胞筛选和其他细胞/组织成像应用。 MHI仪器预计将有利于生物和环境疾病/病原体的检测和鉴定，以及研究生和本科生的教育。影响包括快速高分辨率（高通量/高内容）细胞成像，用于早期检测环境病原体和生物危害，以及导致阐明生物分子影响细胞和组织功能的机制的研究。换句话说，MHI可用于快速读取DNA和蛋白质阵列，以及监测和绘制环境和工程复合材料中的化学物质。教育活动包括研究生在先进的细胞处理和成像技术的专业培训，以及研究生和本科生将在使用MHI仪器进行各种生物/生物工程细胞成像研究项目方面接受的多学科培训。

A grant has been awarded to Dr. Ben-Amotz at Purdue University to develop a high speed chemical imaging instrument which will track molecules in living cells. Current chemical imaging instruments are limited by a trade-off between high throughput (speed) and high information content (resolution). This new Multivariate Hyperspectral Imaging (MHI) instrument will overcome these limitations by using a novel data compression strategy to produce a high speed "chemical vision" system. In other words, the MHI instrument will take pictures and movies that reveal where particular molecules are located and how they are moving. In addition to cell imaging, the MHI is expected to be useful for a wide variety of other biological, medical and environmental applications, as further described below. The MHI instrument may be viewed as the first of a new breed of chemical imaging spectrometers which rapidly collects full-spectral images using multivariate data compression. This data compression strategy is related to commonly used music and video data compression methods. However, a key difference is that the MHI uses data compression to speed up the collection of hyperspectral images. This is achieved using a high resolution spatial light modulator (SLM) to rapidly detect full-spectral response functions. The resulting spectral-response intensities may be used either to directly image cells and cell components or to reconstruct complete high resolution spectral images. This hardware data compression strategy is expected to enhance spectral imaging speeds by a factor of 1000, thus facilitating high resolution chemical imaging and dynamics studies of individual live cells, as well as high-throughput/high-content cell screening, and other cell/tissue imaging applications. The MHI instrument is expected to benefit biological and environmental disease/pathogen detection and identification, as well as the education of graduate and undergraduate students. Impacts include fast high-resolution (high-throughput/high-content) imaging of cells for early detection of environmental pathogens and bio-hazards and research leading to elucidation of the mechanisms by which biomolecules influence cellular and tissue function. In other words, the MHI could be used to rapidly read DNA and protein arrays as well as to monitor and map chemicals in environmental and engineering composites. Educational activities include both the specialized training of graduate students in advanced cell handling and imaging techniques, as well as the multi-disciplinary training which graduate and undergraduate students will receive in using MHI instrument for various biological/bio-engineering cell imaging research projects.