Structure of mitochondrial complex I and conformational coupling between electron transfer and proton translocation

质子泵NADH：泛醌氧化还原酶（线粒体复合物I）是一种非常大的膜蛋白，在能量代谢中具有关键功能。我们已经完成了完整的真核生物酶复合物的第一个X-射线晶体学分析，包括40个亚基，质量几乎为1 MDa。未来将需要更高分辨率的结构信息和扩展的功能表征，以充分了解其机制。我们计划结晶和确定最近在我们的实验室获得的稳定和功能的亚复合物的结构，并使用结构引导的荧光能量转移（FRET）的方法来研究功能关键的结构变化，在营业额的复合物I。

Proton pumping NADH:ubiquinone oxidoreductase (mitochondrial complex I) is a very large membrane protein with a key function in energy metabolism. We have accomplished the first X-ray crystallographic analysis of the complete eukaryotic enzyme complex comprising 40 subunits with a mass of almost 1 MDa. Structural information at higher resolution and extended functional characterization will be needed in the future to fully understand its mechanism. We plan to crystallize and determine the structure of stable and functional subcomplexes recently obtained in our laboratory and to use a structure guided fluorescence energy transfer (FRET) approach to investigate functionally critical structural changes during turnover of complex I.